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Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains
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Gómez-Álvarez, Helena, Iturbe, Pablo, Rivero-Buceta, Virginia, Mines, Paul, Bugg, Tim, Nogales, Juan and Díaz, Eduardo (2022) Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains. Bioresource Technology, 346 . 126638. doi:10.1016/j.biortech.2021.126638 ISSN 0960-8524.
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Official URL: https://doi.org/10.1016/j.biortech.2021.126638
Abstract
2,4 pyridine dicarboxylic acid (2,4 PDCA) is an analogue of terephthalate, and hence a target chemical in the field of bio-based plastics. Here, Pseudomonas putida KT2440 strains were engineered to efficiently drive the metabolism of lignin-derived monoaromatics towards 2,4 PDCA in a resting cells-based bioprocess that alleviates growth-coupled limitations and allows biocatalysts recycling. Native β-ketoadipate pathway was blocked by replacing protocatechuate 3,4-dioxygenase by the exogenous LigAB extradiol dioxygenase. Overexpression of pcaK encoding a transporter increased 8-fold 2,4 PDCA productivity from protocatechuate, reaching the highest value reported so far (0.58 g L-1 h-1). Overexpression of the 4-hydroxybenzoate monooxygenase (pobA) speed up drastically the production of 2,4 PDCA from 4-hydroxybenzoate (0.056 g L-1 h-1) or p-coumarate (0.012 g L-1 h-1) achieving values 15-fold higher than those reported with Rhodococcus jostii biocatalysts. 2,4 PDCA was also bioproduced by using soda lignin as feedstock, paving the way for future polymeric lignin valorization approaches.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Biodegradable plastics, Lignin, Pyridine, Pseudomonas -- Biotechnology | |||||||||||||||||||||
Journal or Publication Title: | Bioresource Technology | |||||||||||||||||||||
Publisher: | Elsevier BV | |||||||||||||||||||||
ISSN: | 0960-8524 | |||||||||||||||||||||
Official Date: | February 2022 | |||||||||||||||||||||
Dates: |
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Volume: | 346 | |||||||||||||||||||||
Article Number: | 126638 | |||||||||||||||||||||
DOI: | 10.1016/j.biortech.2021.126638 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Date of first compliant deposit: | 7 January 2022 | |||||||||||||||||||||
Date of first compliant Open Access: | 2 February 2022 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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