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Aminoacyl chain translocation catalysed by a type II thioesterase domain in an unusual non-ribosomal peptide synthetase
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Wang, Shan, Brittain, William D. G., Zhang, Qian, Lu, Zhou, Tong, Ming Him, Wu, Kewen, Kyeremeh, Kwaku, Jenner, Matthew, Yu, Yi, Cobb, Steven L. and Deng, Hai (2022) Aminoacyl chain translocation catalysed by a type II thioesterase domain in an unusual non-ribosomal peptide synthetase. Nature Communications, 13 . 62. doi:10.1038/s41467-021-27512-0 ISSN 2041-1723.
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WRAP-aminoacyl-chain-translocation-catalysed-type-II-thioesterase-domain-unusual-non-ribosomal-peptide-synthetase-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2001Kb) | Preview |
Official URL: https://doi.org/10.1038/s41467-021-27512-0
Abstract
Non-Ribosomal Peptide Synthetases (NRPSs) assemble a diverse range of natural products with important applications in both medicine and agriculture. They consist of several multienzyme subunits that must interact with each other in a highly controlled manner to facilitate efficient chain transfer, thus ensuring biosynthetic fidelity. Several mechanisms for chain transfer are known for NRPSs, promoting structural diversity. Herein, we report the first biochemically characterized example of a type II thioesterase (TEII) domain capable of catalysing aminoacyl chain transfer between thiolation (T) domains on two separate NRPS subunits responsible for installation of a dehydrobutyrine moiety. Biochemical dissection of this process reveals the central role of the TEII-catalysed chain translocation event and expands the enzymatic scope of TEII domains beyond canonical (amino)acyl chain hydrolysis. The apparent co-evolution of the TEII domain with the NRPS subunits highlights a unique feature of this enzymatic cassette, which will undoubtedly find utility in biosynthetic engineering efforts.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||||||||||
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Subjects: | Q Science > QP Physiology T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||||||||||||||||||||||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Enzymes -- Synthesis, Peptides -- Synthesis, Protein microarrays | ||||||||||||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Nature Communications | ||||||||||||||||||||||||||||||||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 2041-1723 | ||||||||||||||||||||||||||||||||||||||||||||||||
Official Date: | 10 January 2022 | ||||||||||||||||||||||||||||||||||||||||||||||||
Dates: |
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Volume: | 13 | ||||||||||||||||||||||||||||||||||||||||||||||||
Article Number: | 62 | ||||||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.1038/s41467-021-27512-0 | ||||||||||||||||||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 19 January 2022 | ||||||||||||||||||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 19 January 2022 | ||||||||||||||||||||||||||||||||||||||||||||||||
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