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Aster repulsion drives short-ranged ordering in the Drosophila syncytial blastoderm
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de-Carvalho, Jorge, Tlili, Sham, Hufnagel, Lars, Saunders, Timothy E. and Telley, Ivo A. (2022) Aster repulsion drives short-ranged ordering in the Drosophila syncytial blastoderm. Development, 149 (2). dev199997. doi:10.1242/dev.199997 ISSN 0950-1991.
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Official URL: https://doi.org/10.1242/dev.199997
Abstract
Biological systems are highly complex, yet notably ordered structures can emerge. During syncytial stage development of the Drosophila melanogaster embryo, nuclei synchronously divide for nine cycles within a single cell, after which most of the nuclei reach the cell cortex. The arrival of nuclei to the cortex occurs with remarkable positional order, which is important for subsequent cellularisation and morphological transformations. Yet, the mechanical principles underlying this lattice-like positional order of nuclei remain untested. Here, utilising quantification of nuclei position and division orientation together with embryo explants we show that short-ranged repulsive interactions between microtubule asters ensure the regular distribution and maintenance of nuclear positions in the embryo. Such ordered nuclear positioning still occurs with the loss of actin caps and even the loss of the nuclei themselves; the asters can self-organise with similar distribution to nuclei in the wild-type embryo. The explant assay enabled us to deduce the nature of the mechanical interaction between pairs of nuclei. We used this to predict how the nuclear division axis orientation changes upon nucleus removal from the embryo cortex, which we confirmed in vivo with laser ablation. Overall, we show that short-ranged microtubule-mediated repulsive interactions between asters are important for ordering in the early Drosophila embryo and minimising positional irregularity.
Item Type: | Journal Article | |||||||||||||||||||||||||||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics | |||||||||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||||||||||||||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Centrosomes, Drosophila melanogaster, Cytoskeleton -- Microbiology | |||||||||||||||||||||||||||||||||
Journal or Publication Title: | Development | |||||||||||||||||||||||||||||||||
Publisher: | The Company of Biologists Ltd. | |||||||||||||||||||||||||||||||||
ISSN: | 0950-1991 | |||||||||||||||||||||||||||||||||
Official Date: | 26 January 2022 | |||||||||||||||||||||||||||||||||
Dates: |
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Volume: | 149 | |||||||||||||||||||||||||||||||||
Number: | 2 | |||||||||||||||||||||||||||||||||
Article Number: | dev199997 | |||||||||||||||||||||||||||||||||
DOI: | 10.1242/dev.199997 | |||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||||||||||||||
Copyright Holders: | © 2022. Published by The Company of Biologists Ltd | |||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 25 February 2022 | |||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 26 January 2023 | |||||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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