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A yeast -two-hybrid screening identifies novel Atg8a-interacting proteins in Drosophila
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Tsapras, Panagiotis and Nezis, Ioannis P. (2022) A yeast -two-hybrid screening identifies novel Atg8a-interacting proteins in Drosophila. Autophagy, 18 (5). pp. 1211-1212. doi:10.1080/15548627.2022.2045535 ISSN 1554-8627.
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WRAP-yeas-two-hybrid-screening-identifies-novel-Atg8a-interacting-proteins-Drosophila-2022.pdf - Accepted Version - Requires a PDF viewer. Download (245Kb) | Preview |
Official URL: https://doi.org/10.1080/15548627.2022.2045535
Abstract
Macroautophagy/autophagy-related protein Atg8/LC3 is important for autophagosome biogenesis and required for selective degradation of various substrates. In our recent study, we performed a yeast two-hybrid screening to identify proteins that interact with Atg8a, the Drosophila homolog of Atg8/LC3. The screening identified several Atg8a-interacting proteins. These proteins include: i) proteins which have already been experimentally verified to bind Atg8a, such as Atg1, DOR, ref(2)P and key (Kenny); ii) proteins for which their mammalian homologs interact with Atg8-family members, like Ank2, Atg4, and Nedd4; and iii) several novel Atg8a-interacting proteins, such as trc/STK38 and Tak1. We showed that Tak1, as well as its co-activator, Tab2, both interact with Atg8a and are substrates for selective autophagic clearance. We also determined that SH3PX1 interacts with Tab2 and is necessary for the effective regulation of the immune-deficiency (IMD) pathway. Our findings suggest a mechanism for the regulatory interactions between Tak1-Tab2-SH3PX1 and Atg8a, which contribute to the fine-tuning of the IMD pathway.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QH Natural history > QH301 Biology | |||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Cell death -- Research, Apoptosis, Drosophila, Proteins -- Analysis, Proteins -- Synthesis | |||||||||||||||
Journal or Publication Title: | Autophagy | |||||||||||||||
Publisher: | Landes Bioscience | |||||||||||||||
ISSN: | 1554-8627 | |||||||||||||||
Official Date: | 28 February 2022 | |||||||||||||||
Dates: |
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Volume: | 18 | |||||||||||||||
Number: | 5 | |||||||||||||||
Page Range: | pp. 1211-1212 | |||||||||||||||
DOI: | 10.1080/15548627.2022.2045535 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Reuse Statement (publisher, data, author rights): | โThis is an Accepted Manuscript of an article published by Taylor & Francis in Autophagy on 28/02/2022, available online: http://www.tandfonline.com/10.1080/15548627.2022.2045535 | |||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||
Date of first compliant deposit: | 21 February 2022 | |||||||||||||||
Date of first compliant Open Access: | 28 February 2023 | |||||||||||||||
RIOXX Funder/Project Grant: |
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