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Genome sequencing and analysis of Trichoderma (Hypocreaceae) isolates exhibiting antagonistic activity against the papaya dieback pathogen, Erwinia mallotivora
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Tamizi, Amin-Asyraf, Mat-Amin, Noriha, Weaver, Jack, Olumakaiye, Richard T., Akbar, Muhamad Afiq, Jin, Sophie, Bunawan, Hamidun and Alberti, Fabrizio (2022) Genome sequencing and analysis of Trichoderma (Hypocreaceae) isolates exhibiting antagonistic activity against the papaya dieback pathogen, Erwinia mallotivora. Journal of Fungi, 8 (3). 246. doi:10.3390/jof8030246 ISSN 2309-608X.
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WRAP-genome-sequencing-analysis-Trichoderma-Hypocreaceae-isolates-exhibiting-antagonistic-activity-against-papaya-dieback-pathogen-Erwinia-mallotivora-Alberti-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1303Kb) | Preview |
Official URL: http://dx.doi.org/10.3390/jof8030246
Abstract
Erwinia mallotivora, the causal agent of papaya dieback disease, is a devastating pathogen that has caused a tremendous decrease in Malaysian papaya export and affected papaya crops in neighbouring countries. A few studies on bacterial species capable of suppressing E. mallotivora have been reported, but the availability of antagonistic fungi remains unknown. In this study, mycelial suspensions from five rhizospheric Trichoderma isolates of Malaysian origin were found to exhibit notable antagonisms against E. mallotivora during co-cultivation. We further characterised three isolates, Trichoderma koningiopsis UKM-M-UW RA5, UKM-M-UW RA6, and UKM-M-UW RA3a, that showed significant growth inhibition zones on plate-based inhibition assays. A study of the genomes of the three strains through a combination of Oxford nanopore and Illumina sequencing technologies highlighted potential secondary metabolite pathways that might underpin their antimicrobial properties. Based on these findings, the fungal isolates are proven to be useful as potential biological control agents against E. mallotivora, and the genomic data opens possibilities to further explore the underlying molecular mechanisms behind their antimicrobial activity, with potential synthetic biology applications.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QK Botany Q Science > QR Microbiology S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Papaya -- Diseases and pests, Dieback , Phytopathogenic microorganisms -- Biological control , Trichoderma , Gram-negative bacteria , Erwinia, Genomes -- Analysis | |||||||||||||||||||||
| Journal or Publication Title: | Journal of Fungi | |||||||||||||||||||||
| Publisher: | MDPI | |||||||||||||||||||||
| ISSN: | 2309-608X | |||||||||||||||||||||
| Official Date: | 28 February 2022 | |||||||||||||||||||||
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| Volume: | 8 | |||||||||||||||||||||
| Number: | 3 | |||||||||||||||||||||
| Article Number: | 246 | |||||||||||||||||||||
| DOI: | 10.3390/jof8030246 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 1 March 2022 | |||||||||||||||||||||
| Date of first compliant Open Access: | 2 March 2022 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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