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Hospital outbreak of carbapenem-resistant Enterobacterales associated with a bla OXA-48 plasmid carried mostly by Escherichia coli ST399
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Ledda, Alice, Cummins, Martina, Shaw, Liam P., Jauneikaite, Elita, Cole, Kevin, Lasalle, Florent, Barry, Deborah, Turton, Jane, Rosmarin, Caryn, Anaraki, Sudy, Wareham, David, Stoesser, Nicole, Paul, John, Manuel, Rohini, Cherian, Benny P. and Didelot, Xavier (2022) Hospital outbreak of carbapenem-resistant Enterobacterales associated with a bla OXA-48 plasmid carried mostly by Escherichia coli ST399. Microbial Genomics, 8 (4). doi:10.1099/mgen.0.000675 ISSN 2057-5858.
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Official URL: https://doi.org/10.1099/mgen.0.000675
Abstract
A hospital outbreak of carbapenem-resistant Enterobacterales was detected by routine surveillance. Whole genome sequencing and subsequent analysis revealed a conserved promiscuous bla OXA-48 carrying plasmid as the defining factor within this outbreak. Four different species of Enterobacterales were involved in the outbreak. Escherichia coli ST399 accounted for 35 of all the 55 isolates. Comparative genomics analysis using publicly available E. coli ST399 genomes showed that the outbreak E. coli ST399 isolates formed a unique clade. We developed a mathematical model of pOXA-48-like plasmid transmission between host lineages and used it to estimate its conjugation rate, giving a lower bound of 0.23 conjugation events per lineage per year. Our analysis suggests that co-evolution between the pOXA-48-like plasmid and E. coli ST399 could have played a role in the outbreak. This is the first study to report carbapenem-resistant E. coli ST399 carrying blaOXA-48 as the main cause of a plasmid-borne outbreak within a hospital setting. Our findings suggest complementary roles for both plasmid conjugation and clonal expansion in the emergence of this outbreak.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QR Microbiology | |||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Science > Statistics |
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SWORD Depositor: | Library Publications Router | |||||||||
Library of Congress Subject Headings (LCSH): | Drug resistance in microorganisms, Enterobacteriaceae, Escherichia coli, Transgenic organisms, Genetic transformation, Plasmids | |||||||||
Journal or Publication Title: | Microbial Genomics | |||||||||
Publisher: | Microbiology Society | |||||||||
ISSN: | 2057-5858 | |||||||||
Official Date: | 20 April 2022 | |||||||||
Dates: |
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Volume: | 8 | |||||||||
Number: | 4 | |||||||||
DOI: | 10.1099/mgen.0.000675 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Copyright Holders: | © 2022 Crown Copyright | |||||||||
Date of first compliant deposit: | 5 May 2022 | |||||||||
Date of first compliant Open Access: | 5 May 2022 | |||||||||
RIOXX Funder/Project Grant: |
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