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An evolutionary conserved detoxification system for membrane lipid–derived peroxyl radicals in Gram-negative bacteria
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Naguib, Marwa, Feldman, Nicolás, Zarodkiewicz, Paulina, Shropshire, Holly, Biamis, Christina, El-Halfawy, Omar M., McCain, Julia, Dezanet, Clément, Décout, Jean-Luc, Chen, Yin, Cosa, Gonzalo and Valvano, Miguel A. (2022) An evolutionary conserved detoxification system for membrane lipid–derived peroxyl radicals in Gram-negative bacteria. PLoS Biology, 20 (5). e3001610. doi:10.1371/journal.pbio.3001610 ISSN 1545-7885.
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WRAP-evolutionary-conserved-detoxification-system-membrane-lipid–derived-peroxyl-radicals-Gram-negative-bacteria-Chen-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2267Kb) | Preview |
Official URL: https://doi.org/10.1371/journal.pbio.3001610
Abstract
How double-membraned Gram-negative bacteria overcome lipid peroxidation is virtually unknown. Bactericidal antibiotics and superoxide ion stress stimulate the transcription of the Burkholderia cenocepacia bcnA gene that encodes a secreted lipocalin. bcnA gene orthologs are conserved in bacteria and generally linked to a conserved upstream gene encoding a cytochrome b561 membrane protein (herein named lcoA, lipocalin-associated cytochrome oxidase gene). Mutants in bcnA, lcoA, and in a gene encoding a conserved cytoplasmic aldehyde reductase (peroxidative stress-associated aldehyde reductase gene, psrA) display enhanced membrane lipid peroxidation. Compared to wild type, the levels of the peroxidation biomarker malondialdehyde (MDA) increase in the mutants upon exposure to sublethal concentrations of the bactericidal antibiotics polymyxin B and norfloxacin. Microscopy with lipid peroxidation–sensitive fluorescent probes shows that lipid peroxyl radicals accumulate at the bacterial cell poles and septum and peroxidation is associated with a redistribution of anionic phospholipids and reduced antimicrobial resistance in the mutants. We conclude that BcnA, LcoA, and PsrA are components of an evolutionary conserved, hitherto unrecognized peroxidation detoxification system that protects the bacterial cell envelope from lipid peroxyl radicals.
Item Type: | Journal Article | |||||||||||||||||||||||||||
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Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Gram-negative bacteria , Membrane lipids, Membrane lipids -- Peroxidation | |||||||||||||||||||||||||||
Journal or Publication Title: | PLoS Biology | |||||||||||||||||||||||||||
Publisher: | Public Library of Science | |||||||||||||||||||||||||||
ISSN: | 1545-7885 | |||||||||||||||||||||||||||
Official Date: | 17 May 2022 | |||||||||||||||||||||||||||
Dates: |
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Volume: | 20 | |||||||||||||||||||||||||||
Number: | 5 | |||||||||||||||||||||||||||
Article Number: | e3001610 | |||||||||||||||||||||||||||
DOI: | 10.1371/journal.pbio.3001610 | |||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
Date of first compliant deposit: | 18 May 2022 | |||||||||||||||||||||||||||
Date of first compliant Open Access: | 18 May 2022 | |||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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