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Constriction rate modulation can drive cell size control and homeostasis in C. crescentus
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Lambert, Ambroise, Vanhecke, Aster, Archetti, Anna, Holden, Séamus, Schaber, Felix, Pincus, Zachary, Laub, Michael T., Goley, Erin and Manley, Suliana (2018) Constriction rate modulation can drive cell size control and homeostasis in C. crescentus. iScience, 4 . pp. 180-189. doi:10.1016/j.isci.2018.05.020 ISSN 2589-0042.
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Official URL: http://dx.doi.org/10.1016/j.isci.2018.05.020
Abstract
Rod-shaped bacteria typically grow first via sporadic and dispersed elongation along their lateral walls and then via a combination of zonal elongation and constriction at the division site to form the poles of daughter cells. Although constriction comprises up to half of the cell cycle, its impact on cell size control and homeostasis has rarely been considered. To reveal the roles of cell elongation and constriction in bacterial size regulation during cell division, we captured the shape dynamics of Caulobacter crescentus with time-lapse structured illumination microscopy and used molecular markers as cell-cycle landmarks. We perturbed the constriction rate using a hyperconstriction mutant or fosfomycin ([(2R,3S)-3-methyloxiran-2-yl]phosphonic acid) inhibition. We report that the constriction rate contributes to both size control and homeostasis, by determining elongation during constriction and by compensating for variation in pre-constriction elongation on a single-cell basis.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | iScience | ||||||
Publisher: | Cell Press | ||||||
ISSN: | 2589-0042 | ||||||
Official Date: | June 2018 | ||||||
Dates: |
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Volume: | 4 | ||||||
Page Range: | pp. 180-189 | ||||||
DOI: | 10.1016/j.isci.2018.05.020 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) |
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