Cabello-Yeves, Pedro J., Scanlan, David J., Callieri, Cristiana, Picazo, Antonio, Schallenberg, Lena, Huber, Paula, Roda-Garcia, Juan J., Bartosiewicz, Maciej, Belykh, Olga I., Tikhonova, Irina V., Torcello-Requena, Alberto, De Prado, Paula Martin, Millard, Andrew D., Camacho, Antonio, Rodriguez-Valera, Francisco and Puxty, Richard J. (2022) α-cyanobacteria possessing form IA RuBisCO globally dominate aquatic habitats. The ISME Journal, 16 . pp. 2421-2432. doi:10.1038/s41396-022-01282-z ISSN 1751-7362.
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Abstract
RuBisCO (ribulose 1,5-bisphosphate carboxylase/oxygenase) is one the most abundant enzymes on Earth. Virtually all food webs depend on its activity to supply fixed carbon. In aerobic environments, RuBisCO struggles to distinguish efficiently between CO2 and O2. To compensate, organisms have evolved convergent solutions to concentrate CO2 around the active site. The genetic engineering of such inorganic carbon concentrating mechanisms (CCMs) into plants could help facilitate future global food security for humankind. In bacteria, the carboxysome represents one such CCM component, of which two independent forms exist: α and β. Cyanobacteria are important players in the planet’s carbon cycle and the vast majority of the phylum possess a β-carboxysome, including most cyanobacteria used as laboratory models. The exceptions are the exclusively marine Prochlorococcus and Synechococcus that numerically dominate open ocean systems. However, the reason why marine systems favor an α-form is currently unknown. Here, we report the genomes of 58 cyanobacteria, closely related to marine Synechococcus that were isolated from freshwater lakes across the globe. We find all these isolates possess α-carboxysomes accompanied by a form 1A RuBisCO. Moreover, we demonstrate α-cyanobacteria dominate freshwater lakes worldwide. Hence, the paradigm of a separation in carboxysome type across the salinity divide does not hold true, and instead the α-form dominates all aquatic systems. We thus question the relevance of β-cyanobacteria as models for aquatic systems at large and pose a hypothesis for the reason for the success of the α-form in nature.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
| Library of Congress Subject Headings (LCSH): | Cyanobacteria, Water -- Microbiology, Microbial ecology |
| Journal or Publication Title: | The ISME Journal |
| Publisher: | Nature Publishing Group |
| ISSN: | 1751-7362 |
| Official Date: | October 2022 |
| Dates: | Date Event October 2022 Published 18 July 2022 Available 28 June 2022 Accepted 25 January 2022 Submitted |
| Volume: | 16 |
| Page Range: | pp. 2421-2432 |
| DOI: | 10.1038/s41396-022-01282-z |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Open Access (Creative Commons open licence) |
| Date of first compliant deposit: | 2 August 2022 |
| Date of first compliant Open Access: | 3 August 2022 |
| RIOXX Funder/Project Grant: | Project/Grant ID RIOXX Funder Name Funder ID UNSPECIFIED [MES] Ministry of Education and Science of the Russian Federation |
| URI: | https://wrap.warwick.ac.uk/167805/ |
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