The Library
Elucidating the picocyanobacteria salinity divide through ecogenomics of new freshwater isolates
Tools
Cabello-Yeves, Pedro J., Callieri, Cristiana, Picazo, Antonio, Schallenberg, Lena, Huber, Paula, Roda-Garcia, Juan J., Bartosiewicz, Maciej, Belykh, Olga I., Tikhonova, Irina V., Torcello-Requena, Alberto, De Prado, Paula Martin, Puxty, Richard J., Millard, Andrew D., Camacho, Antonio, Rodriguez-Valera, Francisco and Scanlan, David J. (2022) Elucidating the picocyanobacteria salinity divide through ecogenomics of new freshwater isolates. BMC Biology, 20 . 175. doi:10.1186/s12915-022-01379-z ISSN 1741-7007.
|
PDF
WRAP-Elucidating-the-picocyanobacteria-salinity-divide-through-ecogenomics-of-new-freshwater-isolates-Scanlan-22.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (4Mb) | Preview |
Official URL: https://doi.org/10.1186/s12915-022-01379-z
Abstract
Background: Cyanobacteria are the major prokaryotic primary producers occupying a range of aquatic habitats worldwide that differ in levels of salinity, making them a group of interest to study one of the major unresolved conundrums in aquatic microbiology which is what distinguishes a marine microbe from a freshwater one? We address this question using ecogenomics of a group of picocyanobacteria (cluster 5) that have recently evolved to inhabit geographically disparate salinity niches. Our analysis is made possible by the sequencing of 58 new genomes from freshwater representatives of this group that are presented here, representing a 6-fold increase in the available genomic data.
Results: Overall, fresh water strains had larger genomes(≈2.9 Mb) and %GC content (≈64%) compared to brackish (2.69 Mb and 64%) and marine (2.5 Mb and 58.5%) isolates. Genomic novelties/differences across the salinity divide highlighted acidic proteomes and specific salt-adaptation pathways in marine isolates (e.g. osmolytes/compatible solutes-glycine betaine/ggp/gpg/gmg clusters and glycerolipids (glpK/glpA),whilst freshwater strains possessed distinct ion/potassium channels, permeases (aquaporin Z), fatty-acid desaturases and more neutral/basic proteomes. Sulfur, nitrogen, phosphorus, carbon (photosynthesis)or stress tolerance metabolism whilst showing distinct genomic footprints between habitats e.g. different types of transporters, did not obviously translate into major functionality differences between environments. Brackish microbes show a mixture of marine (salt adaptation pathways) and freshwater features, highlighting their transitional nature.
Conclusions: The plethora of freshwater isolates provided here, in terms of trophic status preference and genetic diversity, exemplifies their ability to colonize ecologically diverse waters across the globe. Moreover, a trend towards larger and more flexible/adaptive genomes in fresh water picocyanobacteria may hint at a wider number of ecological niches in this environment compared to the relatively homogeneous marine system.
Item Type: | Journal Article | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QR Microbiology | ||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cyanobacteria, Cyanobacteria -- Ecology, Freshwater ecology, Fresh water | ||||||||||||||||||||||||
Journal or Publication Title: | BMC Biology | ||||||||||||||||||||||||
Publisher: | BioMed Central Ltd. | ||||||||||||||||||||||||
ISSN: | 1741-7007 | ||||||||||||||||||||||||
Official Date: | 8 August 2022 | ||||||||||||||||||||||||
Dates: |
|
||||||||||||||||||||||||
Volume: | 20 | ||||||||||||||||||||||||
Number of Pages: | 24 | ||||||||||||||||||||||||
Article Number: | 175 | ||||||||||||||||||||||||
DOI: | 10.1186/s12915-022-01379-z | ||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
Date of first compliant deposit: | 2 August 2022 | ||||||||||||||||||||||||
Date of first compliant Open Access: | 4 August 2022 | ||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
|
||||||||||||||||||||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year