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Cellular and gene expression patterns associated with root bifurcation in Selaginella
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Motte, Hans, Fang, Tao, Parizot, Boris, Smet, Wouter, Yang, Xilan, Poelmans, Ward, Walker, Liam, Njo, Maria, Bassel, George W. and Beeckman, Tom (2022) Cellular and gene expression patterns associated with root bifurcation in Selaginella. Plant Physiology, 190 (4). pp. 2398-2416. doi:10.1093/plphys/kiac402 ISSN 0032-0889.
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WRAP-cellular-gene-expression-patterns-associated-root-bifurcation-Selaginella-2022.pdf - Accepted Version - Requires a PDF viewer. Download (700Kb) | Preview |
Official URL: https://doi.org/10.1093/plphys/kiac402
Abstract
The roots of lycophytes branch through dichotomy or bifurcation, during which the root apex splits into two daughter roots. This is morphologically distinct from lateral root (LR) branching in the extant euphyllophytes, with LRs developing along the root axis at different distances from the apex. Although the process of root bifurcation is poorly understood, such knowledge can be important, because it may represent an evolutionarily ancient strategy that roots recruited to form new stem cells or meristems. In this study, we examined root bifurcation in the lycophyte Selaginella moellendorffii. We characterized an in vitro developmental time frame based on repetitive apex bifurcations, allowing us to sample different stages of dichotomous root branching and analyze the root meristem and root branching in S. moellendorffii at the microscopic and transcriptomic level. Our results showed that, in contrast to previous assumptions, initial cells (ICs) in the root meristem are mostly not tetrahedral but rather show an irregular shape. Tracking down the early stages of root branching argues for the occurrence of a symmetric division of the single IC, resulting in two apical stem cells that initiate root meristem bifurcation. Moreover, we generated a S. moellendorffii root branching transcriptome that resulted in the delineation of a subset of core meristem genes. The occurrence of multiple putative orthologs of meristem genes in this dataset suggests the presence of conserved pathways in the control of meristem and root stem cell establishment or maintenance.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QK Botany | |||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Selaginella -- Roots -- Physiology, Roots (Botany) -- Development, Meristems | |||||||||||||||||||||
Journal or Publication Title: | Plant Physiology | |||||||||||||||||||||
Publisher: | American Society of Plant Biologists | |||||||||||||||||||||
ISSN: | 0032-0889 | |||||||||||||||||||||
Official Date: | December 2022 | |||||||||||||||||||||
Dates: |
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Volume: | 190 | |||||||||||||||||||||
Number: | 4 | |||||||||||||||||||||
Number of Pages: | 19 | |||||||||||||||||||||
Page Range: | pp. 2398-2416 | |||||||||||||||||||||
DOI: | 10.1093/plphys/kiac402 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Reuse Statement (publisher, data, author rights): | This is a pre-copyedited, author-produced version of an article accepted for publication in Plant Physiology following peer review. The version of record Hans Motte, Tao Fang, Boris Parizot, Wouter Smet, Xilan Yang, Ward Poelmans, Liam Walker, Maria Njo, George W Bassel, Tom Beeckman, Cellular and gene expression patterns associated with root bifurcation in Selaginella, Plant Physiology, 2022;, kiac402, is available online at: https://doi.org/10.1093/plphys/kiac402 | |||||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||
Copyright Holders: | American Society of Plant Biologists | |||||||||||||||||||||
Date of first compliant deposit: | 14 September 2022 | |||||||||||||||||||||
Date of first compliant Open Access: | 27 August 2023 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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