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Proteome adaptations to phosphate starvation in Brassica rapa
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Watts, Jessica (2021) Proteome adaptations to phosphate starvation in Brassica rapa. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3848252
Abstract
Phosphate is a vital macronutrient yet its availability is often a limiting factor which hinders plant growth. A large proportion of phosphate in soils is organic phosphate (Po) which is inaccessible to plants, it needs to be in its inorganic orthophosphate (Pi) form to be accessible. When grown in Pi deficient conditions plants have adapted numerous phosphate starvation responses (PSR), such as an alteration of their proteome and secreted root exudates, to improve their Pi efficiency. Roots will secrete various compounds such as Purple Acid Phosphatases (PAP) and RNases into the surrounding soil to improve the accessibility of Pi from Po sources for plants to utilise. The majority of research on plants molecular responses to Pi deficiency has been performed on Arabidopsis thaliana, with various crop species responses being largely uncharacterised. To gain insight into how the crop species Brassica rapa responds to Pi stress they were grown hydroponically and a PSR was induced. The plants root and shoot proteomes were analysed with mass spectrometry to determine proteomic responses to Pi starvation. When Pi starved, the root exudates displayed a significant increase in abundance of various proteins, including secreted PAPs, RNases, and lipid remodelling proteins which could help to improve the accessibility of Pi and improve Pi uptake efficiency. The root and shoot proteomes also displayed an increased abundance of various phosphatases as well as various proteins involved in phosphate transport, lipid remodelling, and signalling. These changes in protein abundance have not been documented before in response to Pi starvation in B. rapa, therefore provides valuable insights into which proteins play a role in improving Pi efficiency in B. rapa, providing new and interesting candidates that we could utilise to further improve a plants efficiency in Pi deficient conditions.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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Library of Congress Subject Headings (LCSH): | Phosphates, Brassica -- Nutrition, Brassica -- Effect of phosphorus on, Plant proteomics, Plant growth promoting substances, Growth (Plants) | ||||
Official Date: | December 2021 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Jones, Alexandra M. | ||||
Sponsors: | Brewster scholarship | ||||
Format of File: | |||||
Extent: | xv, 169 leaves : colour illustrations, colour charts | ||||
Language: | eng |
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