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A pathway for chitin oxidation in marine bacteria
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Jiang, Wen-Xin, Li, Ping-Yi, Chen, Xiu-Lan, Zhang, Yi-Shuo, Wang, Jing-Ping, Wang, Yan-Jun, Sheng, Qi, Sun, Zhong-Zhi, Qin, Qi-Long, Ren, Xue-Bing, Wang, Peng, Song, Xiao-Yan, Chen, Yin and Zhang, Yu-Zhong (2022) A pathway for chitin oxidation in marine bacteria. Nature Communications, 13 (1). 5899. doi:10.1038/s41467-022-33566-5 ISSN 2041-1723.
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WRAP-A-pathway-for-chitin-oxidation-in-marine-bacteria-Chen-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2082Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/s41467-022-33566-5
Abstract
Oxidative degradation of chitin, initiated by lytic polysaccharide monooxygenases (LPMOs), contributes to microbial bioconversion of crystalline chitin, the second most abundant biopolymer in nature. However, our knowledge of oxidative chitin utilization pathways, beyond LPMOs, is very limited. Here, we describe a complete pathway for oxidative chitin degradation and its regulation in a marine bacterium, Pseudoalteromonas prydzensis. The pathway starts with LPMO-mediated extracellular breakdown of chitin into C1-oxidized chitooligosaccharides, which carry a terminal 2-(acetylamino)−2-deoxy-D-gluconic acid (GlcNAc1A). Transmembrane transport of oxidized chitooligosaccharides is followed by their hydrolysis in the periplasm, releasing GlcNAc1A, which is catabolized in the cytoplasm. This pathway differs from the known hydrolytic chitin utilization pathway in enzymes, transporters and regulators. In particular, GlcNAc1A is converted to 2-keto-3-deoxygluconate 6-phosphate, acetate and NH3 via a series of reactions resembling the degradation of D-amino acids rather than other monosaccharides. Furthermore, genomic and metagenomic analyses suggest that the chitin oxidative utilization pathway may be prevalent in marine Gammaproteobacteria.
Item Type: | Journal Article | |||||||||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Chitin, Marine microbiology, Polysaccharides, Bacteria -- Physiology | |||||||||||||||||||||||||||
Journal or Publication Title: | Nature Communications | |||||||||||||||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||||||||||||||
ISSN: | 2041-1723 | |||||||||||||||||||||||||||
Official Date: | 6 October 2022 | |||||||||||||||||||||||||||
Dates: |
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Volume: | 13 | |||||||||||||||||||||||||||
Number: | 1 | |||||||||||||||||||||||||||
Number of Pages: | 15 | |||||||||||||||||||||||||||
Article Number: | 5899 | |||||||||||||||||||||||||||
DOI: | 10.1038/s41467-022-33566-5 | |||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
Date of first compliant deposit: | 25 October 2022 | |||||||||||||||||||||||||||
Date of first compliant Open Access: | 25 October 2022 | |||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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