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A multi-omics study of tapping and ethylene induction in Hevea brasiliensis
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Dawson, Alexander Takahide Takao (2021) A multi-omics study of tapping and ethylene induction in Hevea brasiliensis. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3853437
Abstract
Hevea brasiliensis, the Par`a rubber tree, is the primary source of natural rubber (NR). NR is harvested through the wounding, or tapping, of rubber trees, and the collection of the latex that flows from the wound. Serial tapping and ethylene stimulation can boost the yield of latex harvested after each tapping, though the mechanisms that underpin this increase in yield have not been fully characterised. This study has generated whole-genome transcriptome and methylome datasets, in order to explore the regulatory mechanisms that underpin latex production in response to tapping and ethylene stimulation. Analyses show key patterns of differential expression across genes of functional significance, including a large number of genes under the control of endogenous-ethylene, whose transcriptional response to tapping is enhanced by exogenous ethylene. This includes numerous DNA methylation relevant genes, indicating an epigenetic role in latex production, and therefore prompting the generation of bisulfite-seq libraries. Collectively, these experiments will provide great insight into the mechanisms underpinning latex production.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history > QH426 Genetics S Agriculture > SB Plant culture |
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Library of Congress Subject Headings (LCSH): | Hevea brasiliensis -- Genetics, Tree tapping, Rubber, Latex, DNA -- Methylation, Ethylene -- Synthesis | ||||
Official Date: | June 2021 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Gutierrez-Marcos, José F. | ||||
Sponsors: | Biotechnology and Biological Sciences Research Council (Great Britain) ; Midlands Integrative Biosciences Training Partnership | ||||
Format of File: | |||||
Extent: | xxviii unnumbered pages, 204 pages : illustrations (mostly colour) | ||||
Language: | eng |
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