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High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers
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Ouyang, Ruochen, Costa, Ana Rita, Cassidy, C. Keith, Otwinowska, Aleksandra, Williams, Vera C. J., Latka, Agnieszka, Stansfeld, Phillip J., Drulis-Kawa, Zuzanna, Briers, Yves, Pelt, Daniël M., Brouns, Stan J. J. and Briegel, Ariane (2022) High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers. Nature Communications, 13 (1). 7241. doi:10.1038/s41467-022-34972-5 ISSN 2041-1723.
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Official URL: https://doi.org/10.1038/s41467-022-34972-5
Abstract
The Klebsiella jumbo myophage ϕKp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein structure prediction methods, molecular simulations, microbiological and machine learning approaches to explore the capsid, tail, and tail fibers of ϕKp24. We determine the structure of the capsid and tail at 4.1 Å and 3.0 Å resolution. We observe the tail fibers are branched and rearranged dramatically upon cell surface attachment. This complex configuration involves fourteen putative tail fibers with depolymerase activity that provide ϕKp24 with the ability to infect a broad panel of capsular polysaccharide (CPS) types of Klebsiella pneumoniae. Our study provides structural and functional insight into how ϕKp24 adapts to the variable surfaces of capsulated bacterial pathogens, which is useful for the development of phage therapy approaches against pan-drug resistant K. pneumoniae strains.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||||||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Klebsiella pneumoniae -- Imaging, Bacteriophages -- Imaging, Drug resistance in microorganisms | ||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Nature Communications | ||||||||||||||||||||||||||||||||||||
Publisher: | Nature Publishing Group UK | ||||||||||||||||||||||||||||||||||||
ISSN: | 2041-1723 | ||||||||||||||||||||||||||||||||||||
Official Date: | 24 November 2022 | ||||||||||||||||||||||||||||||||||||
Dates: |
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Volume: | 13 | ||||||||||||||||||||||||||||||||||||
Number: | 1 | ||||||||||||||||||||||||||||||||||||
Article Number: | 7241 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.1038/s41467-022-34972-5 | ||||||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 19 December 2022 | ||||||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 19 December 2022 | ||||||||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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