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Investigating the phylogeny, interactions, and management of pathogens within the pea foot rot complex
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King, Lisa Marie (2022) Investigating the phylogeny, interactions, and management of pathogens within the pea foot rot complex. PhD thesis, University of Warwick.
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WRAP_Theses_King_2022.pdf - Submitted Version - Requires a PDF viewer. Download (33Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3857766
Abstract
The field pea (Pisum sativum) is an important legume grown for human and animal consumption. In the UK, growth is restricted to eastern England and Scotland, which has led to intensive production and yield declines of up to 40 %. This is attributed to a build-up of fungal and oomycete pathogens involved in the pea foot rot complex (PFRC); Fusarium solani forma speciales (f. sp.) pisi (FSP), F. oxysporum (FO), Didymella pinodella (DP) and Aphanomyces euteiches. Knowledge regarding the genetics, dynamics, and control of these pathogens, in particular for DP, is limited. Therefore, the main aims of this project were to characterise selected PFRC pathogen isolates through multilocus sequencing, better understand PFRC pathogen virulence, dynamics and interactions, and identify biofumigant crops that can suppress PFRC pathogens. Sequencing and multilocus phylogenetic analysis of both DP and F. solani (FS) isolates revealed the lack of diversity within DP isolates from the UK and other countries, while UK FS isolates clustered within a previously published clade of FS isolates from a variety of legume hosts, which challenges the notion that FS formae speciales are specific to single plant species. Positive relationships between pathogen inoculum concentration, pea foot rot disease development and plant mortality were established for FO, FSP and DP using a test-tube based assay, and for DP a glasshouse-based assay. A further test tube assay revealed the additive nature of interactions between FO, FSP and DP when coinoculated in terms of disease development in pea. Preliminary qPCR analysis of root colonisation in this experiment successfully quantified DNA for each of the three pathogens. Biofumigant crop varieties, particularly species of Brassica juncea and Eruca sativa, significantly reduced and inhibited mycelial growth of FO, FSP, DP and AE invitro and significantly supressed DP foot rot in a pot-based glasshouse experiment. Overall, this research has contributed substantial knowledge regarding the phylogeny, disease development and interactions of PFRC pathogens, and the potential of biofumigation as a management strategy.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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Library of Congress Subject Headings (LCSH): | Peas, Peas -- Diseases and pests, Eyespot (Plant disease), Phytopathogenic microorganisms, Phytopathogenic fungi | ||||
Official Date: | January 2022 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Clarkson, John ; Bending, G. D. (Gary D.) | ||||
Sponsors: | Midlands Integrative Biosciences Training Partnership ; Processors & Growers Research Organisation (Great Britain) | ||||
Format of File: | |||||
Extent: | xv, 170 pages : illustrations (some colour) | ||||
Language: | eng |
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