The Library
Multi-centre discriminating concentration determination of broflanilide and potential for cross-resistance to other public health insecticides in Anopheles vector populations
Tools
Portwood, Natalie M., Shayo, Magreth F., Tungu, Patrick K., Mbewe, Njelembo J., Mlay, George, Small, Graham, Snetselaar, Janneke, Kristan, Mojca, Levy, Prisca, Walker, Thomas, Kirby, Matthew J., Kisinza, William, Mosha, Franklin W., Rowland, Mark and Messenger, Louisa A. (2022) Multi-centre discriminating concentration determination of broflanilide and potential for cross-resistance to other public health insecticides in Anopheles vector populations. Scientific Reports, 12 (1). 22359. doi:10.1038/s41598-022-26990-6 ISSN 2045-2322.
|
PDF
WRAP-multi-centre-discriminating-concentration-determination-broflanilide-potential-cross-resistance-other-public-health-insecticides-Anopheles-vector-populations-Walker-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1464Kb) | Preview |
Official URL: https://doi.org/10.1038/s41598-022-26990-6
Abstract
Novel insecticides are urgently needed to control insecticide-resistant populations of Anopheles malaria vectors. Broflanilide acts as a non-competitive antagonist of the gamma-aminobutyric acid receptor and has shown prolonged effectiveness as an indoor residual spraying product (VECTRON T500) in experimental hut trials against pyrethroid-resistant vector populations. This multi-centre study expanded upon initial discriminating concentration testing of broflanilide, using six Anopheles insectary colonies (An. gambiae Kisumu KCMUCo, An. gambiae Kisumu NIMR, An. arabiensis KGB, An. arabiensis SENN, An. coluzzii N’Gousso and An. stephensi SK), representing major malaria vector species, to facilitate prospective susceptibility monitoring of this new insecticide; and investigated the potential for cross-resistance to broflanilide via the A296S mutation associated with dieldrin resistance (rdl). Across all vector species tested, the discriminating concentration for broflanilide ranged between LC99 × 2 = 1.126–54.00 μg/ml or LC95 × 3 = 0.7437–17.82 μg/ml. Lower concentrations of broflanilide were required to induce complete mortality of An. arabiensis SENN (dieldrin-resistant), compared to its susceptible counterpart, An. arabiensis KGB, and there was no association between the presence of the rdl mechanism of resistance and survival in broflanilide bioassays, demonstrating a lack of cross-resistance to broflanilide. Study findings provide a benchmark for broflanilide susceptibility monitoring as part of ongoing VECTRON T500 community trials in Tanzania and Benin.
Item Type: | Journal Article | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QL Zoology Q Science > QP Physiology S Agriculture > SB Plant culture |
|||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Insecticides -- Research, Anopheles , Anopheles -- Insecticide resistance, Vector control, GABA -- Receptors | |||||||||||||||
Journal or Publication Title: | Scientific Reports | |||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||
ISSN: | 2045-2322 | |||||||||||||||
Official Date: | 26 December 2022 | |||||||||||||||
Dates: |
|
|||||||||||||||
Volume: | 12 | |||||||||||||||
Number: | 1 | |||||||||||||||
Article Number: | 22359 | |||||||||||||||
DOI: | 10.1038/s41598-022-26990-6 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 11 January 2023 | |||||||||||||||
Date of first compliant Open Access: | 11 January 2023 | |||||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year