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Detection of streptavidin using liquid crystal based whispering gallery mode microbubble
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Wang, Haonan, Xu, Tianhua, Wang, Ziyihui, Jiang, Junfeng, Liu, Tiegen, Peng, Gang-Ding, Yang, Minghong and Fan, Xinyu (2022) Detection of streptavidin using liquid crystal based whispering gallery mode microbubble. In: SPIE/COS Photonics Asia 2022, Online, 5-11 Dec 2022. Published in: Proceedings of SPIE ; Advanced Sensor Systems and Applications XII, 12321 doi:10.1117/12.2637884 ISSN 0277-786X.
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WRAP-detection-streptavidin-using-liquid-crystal-based-whispering-gallery-mode-microbubble-Xu-2022.pdf - Accepted Version - Requires a PDF viewer. Download (3369Kb) | Preview |
Official URL: http://dx.doi.org/10.1117/12.2637884
Abstract
Protein is a complex chemical substance essential for human survival. Traditional protein detection methods, such as colorimetry, electrochemical analysis, and enzyme-linked immunosorbent assays, have shown good specificity and accuracy for the protein detection. However, all these methods require specialized instruments, and the detection procedures are laborious and time-consuming. As a result, a rapid, sensitive, label-free protein detection method is urgently needed. Herein, we have developed an ultra-sensitive biosensor for the detection of low-concentration protein molecules, employing liquid crystal (LC)-amplified optofluidic resonator. Since the orientations of LCs highly depend on the surface biomolecular binding processes, LCs can be employed to realize the extremely sensitive detection of biomolecules. Immobilized protein molecules interfere with the orientation of LCs by reducing the vertical anchoring force from the alignment layer in which the spectral wavelength shift was monitored as a sensing parameter. A biosensing platform based on an LC-amplified optofluidic whispering gallery mode (WGM) resonator was designed and studied accordingly. Due to the simultaneous interaction of the WGM and the LCs in the optofluidic resonator, the changes caused by the injection of protein molecules will be amplified, resulting in a shift in the resonance wavelength. Total wavelength shifts scale proportionally to the molecular concentrations of the protein within a certain range. The detection limit for streptavidin (SA) can reach as low as the femtometer level, which is significantly higher than the detection limit in the classic polarized optical microscope (POM) method visible with the naked eye. In addition to SA, the LC-based optofluidic resonator can also be applied to detect a variety of protein molecules. Our study demonstrates that LC-amplified optofluidic resonator can provide a novel solution for ultrasensitive real-time characterization of biosensing and biomolecular interactions.
Item Type: | Conference Item (Paper) | ||||||||||||
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Subjects: | Q Science > QP Physiology Q Science > QR Microbiology T Technology > TA Engineering (General). Civil engineering (General) T Technology > TJ Mechanical engineering and machinery T Technology > TK Electrical engineering. Electronics Nuclear engineering T Technology > TS Manufactures |
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||||||
Library of Congress Subject Headings (LCSH): | Proteins -- Analysis, Liquid crystals, Liquid crystal devices, Optofluidics , Streptavidin , Biosensors , Microresonators (Optoelectronics) | ||||||||||||
Journal or Publication Title: | Proceedings of SPIE ; Advanced Sensor Systems and Applications XII | ||||||||||||
Publisher: | S P I E - International Society for Optical Engineering | ||||||||||||
ISSN: | 0277-786X | ||||||||||||
Book Title: | Advanced Sensor Systems and Applications XII | ||||||||||||
Official Date: | 19 December 2022 | ||||||||||||
Dates: |
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Volume: | 12321 | ||||||||||||
DOI: | 10.1117/12.2637884 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Reuse Statement (publisher, data, author rights): | Copyright (2022) Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited. | ||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
Date of first compliant deposit: | 4 January 2023 | ||||||||||||
Date of first compliant Open Access: | 4 January 2023 | ||||||||||||
RIOXX Funder/Project Grant: |
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Conference Paper Type: | Paper | ||||||||||||
Title of Event: | SPIE/COS Photonics Asia 2022 | ||||||||||||
Type of Event: | Conference | ||||||||||||
Location of Event: | Online | ||||||||||||
Date(s) of Event: | 5-11 Dec 2022 | ||||||||||||
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