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Characterisation of an unusual cysteine pair in the Rieske carnitine monooxygenase CntA catalytic site
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Quareshy, Mussa, Shanmugam, Muralidharan, Cameron, Alexander, Bugg, Tim and Chen, Yin (2023) Characterisation of an unusual cysteine pair in the Rieske carnitine monooxygenase CntA catalytic site. The FEBS Journal, 290 (11). pp. 2939-2953. doi:10.1111/febs.16722 ISSN 1742-464X.
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The FEBS Journal - 2023 - Quareshy - Characterisation of an unusual cysteine pair in the Rieske carnitine monooxygenase.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3298Kb) | Preview |
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WRAP-characterisation-unusual-cysteine-pair-Rieske-carnitine-monooxygenase-CntA-catalytic-site-2023.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (5Mb) |
Official URL: https://doi.org/10.1111/febs.16722
Abstract
Rieske monooxygenases undertake complex catalysis integral to marine, terrestrial and human gut-ecosystems. Group-I to -IV Rieske monooxygenases accept aromatic substrates and have well-characterised catalytic mechanisms. Nascent to our understanding are Group-V members catalysing the oxidation/breakdown of quaternary ammonium substrates. Phylogenetic analysis of Group V highlights a cysteine residue-pair adjacent to the mononuclear Fe active site with no established role. Following our elucidation of the carnitine monooxygenase CntA structure, we probed the function of the cysteine pair Cys206/Cys209. Utilising biochemical and biophysical techniques, we found the cysteine residues do not play a structural role nor influence the electron transfer pathway, but rather are used in a nonstoichiometric role to ensure the catalytic iron centre remains in an Fe(II) state.
Item Type: | Journal Article | ||||||||||||
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Alternative Title: | |||||||||||||
Subjects: | Q Science > QP Physiology | ||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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Library of Congress Subject Headings (LCSH): | Carnitine, Carnitine deficiency, Carnitine -- Physiological effect, Cysteine proteinases | ||||||||||||
Journal or Publication Title: | The FEBS Journal | ||||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||
ISSN: | 1742-464X | ||||||||||||
Official Date: | June 2023 | ||||||||||||
Dates: |
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Volume: | 290 | ||||||||||||
Number: | 11 | ||||||||||||
Page Range: | pp. 2939-2953 | ||||||||||||
DOI: | 10.1111/febs.16722 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 6 January 2023 | ||||||||||||
Date of first compliant Open Access: | 14 February 2023 | ||||||||||||
RIOXX Funder/Project Grant: |
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