Maguire, Rosanne (2022) Strategic and responsive breeding of common bean varieties (Phaseolus vulgaris L.) for the UK market. PhD thesis, University of Warwick.

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Abstract

Regional adaptation and end-use potential for dry common beans (Phaseolus vulgaris L.) provides an opportunity for sustainable innovation in the UK agrifood system to support the ‘Great Food Transformation’ required for addressing global problems in nutrition and environmental degradation caused by food production.

In this work, molecular genetic methods were applied for both responsive and strategic disease resistance breeding objectives of the Warwick dry bean breeding programme. This includes confirming predictions by J Taylor for genetically distinct sources of recessive halo blight resistance in two P. vulgaris accessions from Africa using an allelism test in one example and genotyping-by-sequencing to map resistance in the second example.

In response to a novel virus outbreak in trial fields, this research applied high throughput sequencing for viral diagnostics and detected the pathogenic potyvirus Bean yellow mosaic virus (BYMV) using total RNA sequencing and metagenomic analyses. A 1.5 Mb mapping interval was defined for a major effect recessive resistance locus to BYMV in the Warwick pre-breeding material on P. vulgaris chromosome 3. Furthermore, whole genome resequencing of parental lines revealed polymorphisms in candidate genes for both halo blight resistance (HB4.2 locus defined by Tock et al., 2017) and the novel BYMV resistance mapped here, as well as providing insights into the relatedness of one parent lines to an English heirloom variety, Ireland Creek Annie.

Additionally, through the lens of consumer behaviour research, consumer attitudes towards pulses and interest in adding homegrown white haricot beans to the UK diet, particularly as a dry ingredient, was investigated. The methods applied to provide this foundational evidence include a systematic literature review examining perceptions and attitudes to pulses, and an online consumer behaviour experiment that investigated effects of packaging country-of-origin and cooking-time messages had on consumer perceptions of dry white haricot beans.

Item Type:	Thesis (PhD)
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany Q Science > QR Microbiology > QR355 Virology S Agriculture > SB Plant culture
Library of Congress Subject Headings (LCSH):	Common bean, Common bean -- Genetic aspects, Common bean -- Disease and pest resistance, Bean yellow mosaic virus
Official Date:	May 2022
Dates:	Date Event May 2022 UNSPECIFIED
Institution:	University of Warwick
Theses Department:	School of Life Sciences
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Holub, E. B.
Sponsors:	Midlands Integrative Biosciences Training Partnership ; Biotechnology and Biological Sciences Research Council (Great Britain)
Format of File:	pdf
Extent:	xvii, 251 pages : illustrations (some colour)
Language:	eng

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