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Molecular mechanism for hepatic glycerolipid partitioning of n-6/n-3 fatty acid ratio in an obese animal biomodels
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Zammit, Victor A. and Park, Sang-O. (2023) Molecular mechanism for hepatic glycerolipid partitioning of n-6/n-3 fatty acid ratio in an obese animal biomodels. International Journal of Molecular Sciences, 24 (2). 1576. doi:10.3390/ijms24021576 ISSN 1422-0067.
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Official URL: http://doi.org/10.3390/ijms24021576
Abstract
The n-6/n-3 metabolic pathway associated with hepatic glycerolipid portioning plays a key role in preventing obesity. In this nutrition metabolism study, we used in vivo monitoring techniques with 40 obese male Sprague-Dawley strain rats attached with jugular-vein cannula after obesity was induced by a high-fat diet to determine the molecular mechanism associated with hepatic glycerolipid partitioning involving the n-6/n-3 metabolic pathway. Rats were randomly assigned to four groups (10 animals per group), including one control group (CON, n-6/n-3 of 71:1) and three treatment groups (n-6/n-3 of 4:1, 15:1 and 30:1). They were fed with experimental diets for 60 days. Incorporation rates of [14C]-labeling lipid into glycerolipid in the liver were 28.87–37.03% in treatment groups fed with diets containing an n-6/n-3 ratio of 4:1, 15:1 and 30:1, which were significantly (p < 0.05) lower than that in the CON (40.01%). However, 14CO2 emission % of absorbed dose showed the opposite trend. It was significantly (p < 0.05) higher in a treatment groups (n-6/n-3 of 4:1, 15:1 and 30:1, 30.35–45.08%) than in CON (27.71%). Regarding the metabolic distribution of glycerolipid to blood from livers, phospholipid/total glycerolipid (%) was significantly (p < 0.05) lower in CON at 11.04% than in treatment groups at 18.15% to 25.15%. Moreover, 14CO2/[14C]-total glycerolipid (%) was significantly (p < 0.05) higher in treatment groups at 44.16–78.50% than in CON at 39.50%. Metabolic distribution of fatty acyl moieties flux for oxidation and glycerolipid synthesis in the liver were significantly (p < 0.05) better in order of 4:1 > 15:1 > 30:1 than in the CON. Our data demonstrate that n-6/n-3 of 4:1 could help prevent obesity by controlling the mechanism of hepatic partitioning through oxidation and esterification of glycerolipid in an obese animal biomodel.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QL Zoology Q Science > QP Physiology R Medicine > RC Internal medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||
Library of Congress Subject Headings (LCSH): | Lipids, Lipids -- Metabolism , Animal models in research , Obesity -- Prevention | ||||||
Journal or Publication Title: | International Journal of Molecular Sciences | ||||||
Publisher: | M D P I AG | ||||||
ISSN: | 1422-0067 | ||||||
Official Date: | 13 January 2023 | ||||||
Dates: |
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Volume: | 24 | ||||||
Number: | 2 | ||||||
Article Number: | 1576 | ||||||
DOI: | 10.3390/ijms24021576 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 1 March 2023 | ||||||
Date of first compliant Open Access: | 1 March 2023 | ||||||
RIOXX Funder/Project Grant: |
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