The Library
Studying nuclear dynamics in response to actin disruption in planta
Tools
McKenna, Joseph F. and Graumann, Katja (2023) Studying nuclear dynamics in response to actin disruption in planta. In: The Plant Cytoskeleton. Methods in Molecular Biology, 2604 (2604). Springer, pp. 203-214. ISBN 9781071628669
PDF
WRAP-Studying-nuclear-dynamics-in-response-to-actin-disruption-in-planta-McKenna-2023.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only until 12 February 2025. Contact author directly, specifying your specific needs. - Requires a PDF viewer. Download (607Kb) |
Official URL: https://doi.org/10.1007/978-1-0716-2867-6_15
Abstract
The plant nucleus and the actin cytoskeleton are intimately connected. The actin cytoskeleton is pivotal for nuclear positioning, shape, and dynamics. These properties of the nucleus are important for its functions during normal development and in response to external cues such as biotic and abiotic stresses. Moreover, we know that there is a direct physical connection between the actin cytoskeleton and the nucleus which spans the double-membraned nuclear envelope into the nuclear lamina, and this connection is called the linker of nucleoskeleton and cytoskeleton (LINC) complex. Recently a role for actin in regulating inter-nuclear organization via the control of nuclear invaginations has emerged. Therefore, a detailed understanding of nuclear shape, organization, and dynamics and the techniques used to measure and quantify these metrics will allow us to determine and further understand the contribution made by actin to these parameters. The protocols described here will allow researchers to determine the circularity index of a nucleus, quantify nuclear deformations, and determine dynamics of nuclei within plant cells.
Item Type: | Book Item | ||||||
---|---|---|---|---|---|---|---|
Subjects: | Q Science > QK Botany | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
SWORD Depositor: | Library Publications Router | ||||||
Library of Congress Subject Headings (LCSH): | Actin, Cell nuclei, Plant cells and tissues -- Research, Plant cytoskeleton, Confocal microscopy | ||||||
Series Name: | Methods in Molecular Biology | ||||||
Journal or Publication Title: | Methods in molecular biology (Clifton, N.J.) | ||||||
Publisher: | Springer | ||||||
ISBN: | 9781071628669 | ||||||
ISSN: | 1940-6029 | ||||||
Book Title: | The Plant Cytoskeleton | ||||||
Official Date: | 12 February 2023 | ||||||
Dates: |
|
||||||
Volume: | 2604 | ||||||
Number: | 2604 | ||||||
Page Range: | pp. 203-214 | ||||||
DOI: | 10.1007/978-1-0716-2867-6_15 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Free Access (unspecified licence, 'bronze OA') | ||||||
Date of first compliant deposit: | 22 March 2023 |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |