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Application of Rhodococcus jostii RHA1 glycolate oxidase as an efficient accessory enzyme for lignin conversion by bacterial Dyp peroxidase enzymes
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Alruwaili, Awatif, Rashid, Goran M. M. and Bugg, Tim (2023) Application of Rhodococcus jostii RHA1 glycolate oxidase as an efficient accessory enzyme for lignin conversion by bacterial Dyp peroxidase enzymes. Green Chemistry, 25 (9). pp. 3549-3560. doi:10.1039/D3GC00475A ISSN 1463-9262.
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Official URL: https://doi.org/10.1039/D3GC00475A
Abstract
Lignin oxidation by bacterial dye-decolorizing peroxidase enzymes requires hydrogen peroxide as a co-substrate, an unstable and corrosive oxidant. We have identified a glycolate oxidase enzyme from Rhodococcus jostii RHA1 that can couple effectively at pH 6.5 with DyP peroxidase enzymes from Agrobacterium sp. or Comamonas testosteroni to oxidise lignin substrates without addition of hydrogen peroxide. Rhodococcus jostii RHA1 glycolate oxidase (RjGlOx) has activity for oxidation of a range of α-ketoaldehyde and α-hydroxyacid substrates, and is also active for oxidation of hydroxymethylfurfural (HMF) to furandicarboxylic acid. The combination of RjGlOx with Agrobacterium sp. DyP or C. testosteroni DyP generated new and enhanced amounts of low molecular weight aromatic products from organosolv lignin substrates, and was able to generate high-value products from treatment of lignin residue from cellulosic biofuel production, and from a polymeric humin substrate.
Item Type: | Journal Article | ||||||||
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Alternative Title: | |||||||||
Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Lignin, Lignin -- Oxidation, Enzymes, Oxidases, Hydrogen -- Biotechnology, Lignocellulose -- Biotechnology, Peroxidase | ||||||||
Journal or Publication Title: | Green Chemistry | ||||||||
Publisher: | Royal Society of Chemistry | ||||||||
ISSN: | 1463-9262 | ||||||||
Official Date: | 7 May 2023 | ||||||||
Dates: |
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Volume: | 25 | ||||||||
Number: | 9 | ||||||||
Page Range: | pp. 3549-3560 | ||||||||
DOI: | 10.1039/D3GC00475A | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 12 April 2023 | ||||||||
Date of first compliant Open Access: | 20 July 2023 | ||||||||
RIOXX Funder/Project Grant: |
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