The Library
A subset of highly responsive transcription factors upon tomato infection by pepino mosaic virus
Tools
Tsitsekian, Dikran, Daras, Gerasimos, Templalexis, Dimitris, Avgeri, Fengoula, Lotos, Leonidas, Orfanidou, Chrysoula G., Ntoukakis, Vardis, Maliogka, Varvara I. and Rigas, Stamatis (2023) A subset of highly responsive transcription factors upon tomato infection by pepino mosaic virus. Plant Biology, 25 (4). pp. 529-540. doi:10.1111/plb.13515 ISSN 1435-8603.
|
PDF
WRAP-subset-highly-responsive-transcription-factors-upon-tomato-infection-pepino-mosaic-virus-Ntoukakis-2023.pdf - Accepted Version - Requires a PDF viewer. Download (4Mb) | Preview |
Official URL: http://doi.org/10.1111/plb.13515
Abstract
Plants have evolved well-tuned surveillance systems, including complex defence mechanisms, to constrain pathogens. TFs are master regulators of host molecular responses against plant pathogens. While PepMV constitutes a major threat to the global tomato production, there is still a lack of information on the key TFs that regulate host responses to this virus.
A combinatorial research approach was applied relying on tomato transcriptome analysis, RT-qPCR validation, phylogenetic classification, comparative analysis of structural features, cis-regulatory element mining and in silico co-expression analysis to identify a set of 11 highly responsive TFs involved in the regulation of host responses to PepMV.
An endemic PepMV isolate, generating typical mosaic symptoms, modified expression of ca. 3.3% of tomato genes, resulting in 1,120 DEGs. Functional classification of 502 upregulated DEGs revealed that photosynthesis, carbon fixation and gene silencing were widely affected, whereas 618 downregulated genes had an impact mainly on plant defence and carotenoid biosynthesis. Strikingly, all 11 highly responsive TFs carried abiotic stress response cis-regulatory elements, whereas five of them were better aligned with rice than with Arabidopsis gene homologues, suggesting that plant responses against viruses may predate divergence into monocots and dicots. Interestingly, tomato C2H2 family TFs, ZAT1-like and ZF2, may have distinct roles in plant defence due to opposite response patterns, similar to their Arabidopsis ZAT10 and ZAT12 homologues.
These highly responsive TFs provide a basis to study in-depth molecular responses of the tomato–PepMV pathosystem, providing a perspective to better comprehend viral infections.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QK Botany Q Science > QR Microbiology S Agriculture > SB Plant culture |
||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Tomatoes -- Diseases and pests -- Research, Nucleotide sequence, Transcription factors -- Research, Zinc-finger proteins, Mosaic viruses | ||||||||
Journal or Publication Title: | Plant Biology | ||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||
ISSN: | 1435-8603 | ||||||||
Official Date: | June 2023 | ||||||||
Dates: |
|
||||||||
Volume: | 25 | ||||||||
Number: | 4 | ||||||||
Page Range: | pp. 529-540 | ||||||||
DOI: | 10.1111/plb.13515 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Re-use Statement: | This is the pre-peer reviewed version of the following article: Tsitsekian, D., Daras, G., Templalexis, D., Avgeri, F., Lotos, L., Orfanidou, C.G., Ntoukakis, V., Maliogka, V.I. and Rigas, S. (2023), A subset of highly responsive transcription factors upon tomato infection by pepino mosaic virus. Plant Biol J., which has been published in final form at https://doi.org/10.1111/plb.13515. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Copyright Holders: | © 2023 German Society for Plant Sciences, Royal Botanical Society of the Netherlands. | ||||||||
Date of first compliant deposit: | 19 April 2023 | ||||||||
Date of first compliant Open Access: | 20 March 2024 | ||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year