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Chemically induced extracellular ice nucleation reduces intracellular ice formation enabling 2D and 3D cellular cryopreservation
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Murray, Kathryn A., Gao, Yanan, Griffiths, Christopher A., Kinney, Nina L. H., Guo, Qiongyu, Gibson, Matthew I. and Whale, Thomas F. (2023) Chemically induced extracellular ice nucleation reduces intracellular ice formation enabling 2D and 3D cellular cryopreservation. JACS Au . doi:10.1021/jacsau.3c00056 (In Press)
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Official URL: https://doi.org/10.1021/jacsau.3c00056
Abstract
3D cell assemblies such as spheroids reproduce the in vivo state more accurately than traditional 2D cell monolayers and are emerging as tools to reduce or replace animal testing. Current cryopreservation methods are not optimized for complex cell models, hence they are not easily banked and not as widely used as 2D models. Here we use soluble ice nucleating polysaccharides to nucleate extracellular ice and dramatically improve spheroid cryopreservation outcomes. This protects the cells beyond using DMSO alone, and with the major advantage that the nucleators function extracellularly and hence do not need to permeate the 3D cell models. Critical comparison of suspension, 2D and 3D cryopreservation outcomes demonstrated that warm-temperature ice nucleation reduces the formation of (fatal) intracellular ice, and in the case of 2/3D models this reduces propagation of ice between adjacent cells. This demonstrates that extracellular chemical nucleators could revolutionize the banking and deployment of advanced cell models.
Item Type: | Journal Item | |||||||||||||||||||||
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Subjects: | Q Science > QC Physics Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cells -- Cryopreservation, Ice nuclei , Polysaccharides , Cryobiology | |||||||||||||||||||||
Journal or Publication Title: | JACS Au | |||||||||||||||||||||
Publisher: | American Chemical Society | |||||||||||||||||||||
ISSN: | 2691-3704 | |||||||||||||||||||||
Official Date: | 25 April 2023 | |||||||||||||||||||||
Dates: |
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DOI: | 10.1021/jacsau.3c00056 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | In Press | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Date of first compliant deposit: | 12 May 2023 | |||||||||||||||||||||
Date of first compliant Open Access: | 12 May 2023 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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