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Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay
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Acford-Palmer, Holly, Phelan, Jody E., Tadesse, Fitsum G., Kristan, Mojca, Collins, Emma, Spadar, Anton, Walker, Thomas, Bousema, Teun, Messenger, Louisa A., Clark, Taane G. and Campino, Susana (2023) Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay. Scientific Reports, 13 (1). 5612. doi:10.1038/s41598-023-32336-7 ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/s41598-023-32336-7
Abstract
Since its first detection in 2012 in Djibouti, Anopheles stephensi has invaded and established in the Horn of Africa, and more recently Nigeria. The expansion of this vector poses a significant threat to malaria control and elimination efforts. Integrated vector management is the primary strategy used to interrupt disease transmission; however, growing insecticide resistance is threatening to reverse gains in global malaria control. We present a next-generation amplicon-sequencing approach, for high-throughput monitoring of insecticide resistance genes (ace1, GSTe2, vgsc and rdl), species identification and characterization of genetic diversity (its2 and cox1) in An. stephensi. Ninety-five An. stephensi mosquitoes, collected in Ethiopia, were screened, identifying 104 SNPs, including the knock-down mutation L958F (L1014F in Musca domestica), and for the first time in this vector species, the A296S substitution (A301S in Drosophila melanogaster) in the rdl locus. Two other amino acid substitutions (ace1-N177D, GSTe2-V189L) were also identified but have not been previously implicated in insecticide resistance. Genetic diversity in the mitochondrial cox1 gene revealed shared haplotypes between Ethiopian An. stephensi with samples from Pakistan, Sudan, and Djibouti. Overall, we present a reliable, cost-effective strategy using amplicon-sequencing to monitor known insecticide resistance mutations, with the potential to identify new genetic variants, to assist in the high-throughput surveillance of insecticide resistance in An. stephensi populations.
Item Type: | Journal Article | ||||||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QL Zoology S Agriculture > SB Plant culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Anopheles , Mosquitoes -- Insecticide resistance, Insecticide resistance , Genetic regulation , Genomes -- Analysis, Malaria -- Prevention | ||||||||||||||||||||||||
Journal or Publication Title: | Scientific Reports | ||||||||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||||||||
ISSN: | 2045-2322 | ||||||||||||||||||||||||
Official Date: | 5 April 2023 | ||||||||||||||||||||||||
Dates: |
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Volume: | 13 | ||||||||||||||||||||||||
Number: | 1 | ||||||||||||||||||||||||
Article Number: | 5612 | ||||||||||||||||||||||||
DOI: | 10.1038/s41598-023-32336-7 | ||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
Date of first compliant deposit: | 10 May 2023 | ||||||||||||||||||||||||
Date of first compliant Open Access: | 12 May 2023 | ||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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