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Detrimental effects of RNAi : a cautionary note on its use in Drosophila ageing studies
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Cotterill, Sue, Alic, Nazif, Hoddinott, Matthew P., Foley, Andrea, Slack, Cathy, Piper, Matthew D. W. and Partridge, Linda (2012) Detrimental effects of RNAi : a cautionary note on its use in Drosophila ageing studies. PLoS One, 7 (9). e45367. doi:10.1371/journal.pone.0045367 ISSN 1932-6203.
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Official URL: http://doi.org/10.1371/journal.pone.0045367
Abstract
RNA interference (RNAi) provides an important tool for gene function discovery. It has been widely exploited in Caenorhabditis elegans ageing research because it does not appear to have any non-specific effects on ageing-related traits in that model organism. We show here that ubiquitous, adult-onset activation of the RNAi machinery, achieved by expressing a double stranded RNA targeting GFP or lacZ for degradation, or by increasing expression of Dicer substantially reduces lifespan in Drosophila melanogaster. Induction of GFPRNAi construct also alters the response of lifespan to nutrition, exacerbating the lifespan-shortening effects of food containing a high quantity of yeast. Our study indicates that activation of the RNAi machinery may have sequence-independent side-effects on lifespan, and that caution needs to be exercised when employing ubiquitous RNAi in Drosophila ageing studies. However, we also show that RNAi restricted to certain tissues may not be detrimental to lifespan.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QL Zoology Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Drosophila melanogaster, Aging , RNA interference, Gene silencing | |||||||||||||||
Journal or Publication Title: | PLoS One | |||||||||||||||
Publisher: | Public Library of Science | |||||||||||||||
ISSN: | 1932-6203 | |||||||||||||||
Official Date: | 17 September 2012 | |||||||||||||||
Dates: |
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Volume: | 7 | |||||||||||||||
Number: | 9 | |||||||||||||||
Article Number: | e45367 | |||||||||||||||
DOI: | 10.1371/journal.pone.0045367 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
Date of first compliant deposit: | 18 May 2023 | |||||||||||||||
Date of first compliant Open Access: | 18 May 2023 | |||||||||||||||
RIOXX Funder/Project Grant: |
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