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Disruption of a DUF247 containing protein alters cell wall polysaccharides and reduces growth in Arabidopsis
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Wannitikul, Pitchaporn, Wattana-Amorn, Pakorn, Sathitnaitham, Sukhita, Sakulkoo, Jenjira, Suttangkakul, Anongpat, Wonnapinij, Passorn, Bassel, George W., Simister, Rachael, Gomez, Leonardo D. and Vuttipongchaikij, Supachai (2023) Disruption of a DUF247 containing protein alters cell wall polysaccharides and reduces growth in Arabidopsis. Plants, 12 (10). 1977. doi:10.3390/plants12101977 ISSN 2223-7747.
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Official URL: https://doi.org/10.3390/plants12101977
Abstract
Plant cell wall biosynthesis is a complex process that requires proteins and enzymes from glycan synthesis to wall assembly. We show that disruption of At3g50120 (DUF247-1), a member of the DUF247 multigene family containing 28 genes in Arabidopsis, results in alterations to the structure and composition of cell wall polysaccharides and reduced growth and plant size. An ELISA using cell wall antibodies shows that the mutants also exhibit ~50% reductions in xyloglucan (XyG), glucuronoxylan (GX) and heteromannan (HM) epitopes in the NaOH fraction and ~50% increases in homogalacturonan (HG) epitopes in the CDTA fraction. Furthermore, the polymer sizes of XyGs and GXs are reduced with concomitant increases in short-chain polymers, while those of HGs and mHGs are slightly increased. Complementation using 35S:DUF247-1 partially recovers the XyG and HG content, but not those of GX and HM, suggesting that DUF247-1 is more closely associated with XyGs and HGs. DUF247-1 is expressed throughout Arabidopsis, particularly in vascular and developing tissues, and its disruption affects the expression of other gene members, indicating a regulatory control role within the gene family. Our results demonstrate that DUF247-1 is required for normal cell wall composition and structure and Arabidopsis growth.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QK Botany | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
SWORD Depositor: | Library Publications Router | ||||||
Library of Congress Subject Headings (LCSH): | Arabidopsis, Pectin, Plant cell walls, Growth (Plants), Xylans, Glucans -- Synthesis, Polysaccharides -- Synthesis | ||||||
Journal or Publication Title: | Plants | ||||||
Publisher: | MDPI AG | ||||||
ISSN: | 2223-7747 | ||||||
Official Date: | 15 May 2023 | ||||||
Dates: |
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Volume: | 12 | ||||||
Number: | 10 | ||||||
Article Number: | 1977 | ||||||
DOI: | 10.3390/plants12101977 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 13 June 2023 | ||||||
Date of first compliant Open Access: | 13 June 2023 |
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