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Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer
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Pei, Xudong, Zhou, Liqi, Huang, Chen, Boyce, Mark, Kim, Judy S., Liberti, Emanuela, Hu, Yiming, Sasaki, Takeo, Nellist, Peter D., Zhang, Peijun, Stuart, David I., Kirkland, Angus I. and Wang, Peng (2023) Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer. Nature Communications, 14 (1). 3027. doi:10.1038/s41467-023-38268-0 ISSN 2041-1723.
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Official URL: https://doi.org/10.1038/s41467-023-38268-0
Abstract
Advances in cryogenic transmission electron microscopy have revolutionised the determination of many macromolecular structures at atomic or near-atomic resolution. This method is based on conventional defocused phase contrast imaging. However, it has limitations of weaker contrast for small biological molecules embedded in vitreous ice, in comparison with cryo-ptychography, which shows increased contrast. Here we report a single-particle analysis based on the use of ptychographic reconstruction data, demonstrating that three dimensional reconstructions with a wide information transfer bandwidth can be recovered by Fourier domain synthesis. Our work suggests future applications in otherwise challenging single particle analyses, including small macromolecules and heterogeneous or flexible particles. In addition structure determination in situ within cells without the requirement for protein purification and expression may be possible.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||
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Subjects: | Q Science > QC Physics | ||||||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | ||||||||||||||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Transmission electron microscopy, Electron microscopy, Rotaviruses, Cytology -- Technique | ||||||||||||||||||||||||||||||
Journal or Publication Title: | Nature Communications | ||||||||||||||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||||||||||||||
ISSN: | 2041-1723 | ||||||||||||||||||||||||||||||
Official Date: | 25 May 2023 | ||||||||||||||||||||||||||||||
Dates: |
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Volume: | 14 | ||||||||||||||||||||||||||||||
Number: | 1 | ||||||||||||||||||||||||||||||
Article Number: | 3027 | ||||||||||||||||||||||||||||||
DOI: | 10.1038/s41467-023-38268-0 | ||||||||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||
Date of first compliant deposit: | 23 June 2023 | ||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 26 June 2023 | ||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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