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High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization
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Lowen, Anice C., McMahon, Andrew, Andrews, Rebecca, Groves, Danielle, Ghani, Sohail V., Cordes, Thorben, Kapanidis, Achillefs N. and Robb, Nicole C. (2023) High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization. PLoS Pathogens, 19 (6). e1011484. doi:10.1371/journal.ppat.1011484 ISSN 1553-7374.
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WRAP-high-throughput-super-resolution-analysis-influenza-virus-pleomorphism-reveals-insights-viral-spatial-organization-Robb-2023.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3663Kb) | Preview |
Official URL: http://dx.doi.org/10.1371/journal.ppat.1011484
Abstract
Many viruses form highly pleomorphic particles. In influenza, virion structure is of interest not only in the context of virus assembly, but also because pleomorphic variations may correlate with infectivity and pathogenicity. We have used fluorescence super-resolution microscopy combined with a rapid automated analysis pipeline, a method well-suited to the study of large numbers of pleomorphic structures, to image many thousands of individual influenza virions; gaining information on their size, morphology and the distribution of membrane-embedded and internal proteins. We observed broad phenotypic variability in filament size, and Fourier transform analysis of super resolution images demonstrated no generalized common spatial frequency patterning of HA or NA on the virion surface, suggesting a model of virus particle assembly where the release of progeny filaments from cells occurs in a stochastic way. We also showed that viral RNP complexes are located preferentially within Archetti bodies when these were observed at filament ends, suggesting that these structures may play a role in virus transmission. Our approach therefore offers exciting new insights into influenza virus morphology and represents a powerful technique that is easily extendable to the study of pleomorphism in other pathogenic viruses.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | R Medicine > RC Internal medicine | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Influenza, Influenza viruses -- Research | ||||||||||||||||||
Journal or Publication Title: | PLoS Pathogens | ||||||||||||||||||
Publisher: | Public Library of Science | ||||||||||||||||||
ISSN: | 1553-7374 | ||||||||||||||||||
Official Date: | 30 June 2023 | ||||||||||||||||||
Dates: |
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Volume: | 19 | ||||||||||||||||||
Number: | 6 | ||||||||||||||||||
Article Number: | e1011484 | ||||||||||||||||||
DOI: | 10.1371/journal.ppat.1011484 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 11 July 2023 | ||||||||||||||||||
Date of first compliant Open Access: | 17 July 2023 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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