The Library
Understanding and exploiting MmyB-like transcriptional activators
Tools
Robertshaw, Daniel (2022) Understanding and exploiting MmyB-like transcriptional activators. PhD thesis, University of Warwick.
|
PDF
WRAP_Theses_Robertshaw_2022.pdf - Submitted Version - Requires a PDF viewer. Download (8Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3921824
Abstract
One of the biggest ongoing challenges facing society and healthcare in the present is the prevalence of antimicrobial resistance. Novel sources of antibiotics are needed desperately to combat this crisis. A potential supply for this could spring from the MmyB-like transcriptional family, of which the methylenomycin cluster activator is a member of.
Three members of this group of activators were deregulated in Streptomcyes coelicolor M145 to investigate their prospective role in secondary metabolism that has been suggested through genome mining. These bacteria were RNA sequenced to study any effects on transcription and coupled with metabolic profile comparisons, which identified novel metabolites not seen before, alluding to some involvement of these genes in the secondary metabolism of S. coelicolor.
Bioinformatic analysis of the Streptomyces coelicolor MmyB-like activators was performed with support from the RNA sequencing to identify a potential conserved sequence within the promoters of this transcriptional family that may be the target of autoregulation.
Further members of this transcriptional family were identified in Rhodococcus species, a criminally understudied genus, with a high potential for secondary metabolism. Two of the newly discovered MmyB-like activators were overexpressed in R. jostii RHA1 and R. erythropolis PR4 and underwent metabolic comparison to look for secondary metabolites, although none were found.
This family of transcriptional activators needs to be studied further as the widespread presence of these genes across actinomycetes could be a catalyst for natural product discovery and the beginning of the fight back against antimicrobial resistance.
Item Type: | Thesis (PhD) | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology |
||||
Library of Congress Subject Headings (LCSH): | Drug resistance in microorganisms, Streptomyces coelicolor -- Genetic aspects, Transcriptional activation, Bioinformatics, Antibiotics | ||||
Official Date: | May 2022 | ||||
Dates: |
|
||||
Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Corre, Christophe | ||||
Sponsors: | Midlands Integrative Biosciences Training Partnership ; Biotechnology and Biological Sciences Research Council (Great Britain) | ||||
Format of File: | |||||
Extent: | xx, 154 pages : colour illustrations, colour charts | ||||
Language: | eng |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |