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The transcription factor NAC102 confers cadmium tolerance by regulating WAKL11 expression and cell wall pectin metabolism in Arabidopsis
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Han, Guang Hao, Huang, Ru Nan, Hong, Li Hong, Xu, Jia Xi, Hong, Yi Guo, Wu, Yu Huan and Chen, Wei Wei (2023) The transcription factor NAC102 confers cadmium tolerance by regulating WAKL11 expression and cell wall pectin metabolism in Arabidopsis. Journal of Integrative Plant Biology, 65 (10). pp. 2262-2278. doi:10.1111/jipb.13557 ISSN 1672-9072.
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Official URL: https://doi.org/10.1111/jipb.13557
Abstract
Cadmium (Cd) toxicity severely limits plant growth and development. Moreover, Cd accumulation in vegetables, fruits, and food crops poses health risks to animals and humans. Although the root cell wall has been implicated in Cd stress in plants, whether Cd binding by cell wall polysaccharides contributes to tolerance remains controversial, and the mechanism underlying transcriptional regulation of cell wall polysaccharide biosynthesis in response to Cd stress is unknown. Here, we functionally characterized an Arabidopsis thaliana NAC-type transcription factor, NAC102, revealing its role in Cd stress responses. Cd stress rapidly induced accumulation of NAC102.1, the major transcript encoding functional NAC102, especially in the root apex. Compared to wild-type plants, a nac102 mutant exhibited enhanced Cd sensitivity, whereas NAC102.1-overexpressing plants displayed the opposite phenotype. Furthermore, NAC102 localizes to the nucleus, binds directly to the promoter of WALL-ASSOCIATED KINASE-LIKE PROTEIN11 (WAKL11), and induces transcription, thereby facilitating pectin degradation and decreasing Cd binding by pectin. Moreover, WAKL11 overexpression restored Cd tolerance in nac102 mutants to the wild-type levels, which was correlated with a lower pectin content and lower levels of pectin-bound Cd. Taken together, our work shows that the NAC102-WAKL11 module regulates cell wall pectin metabolism and Cd binding, thus conferring Cd tolerance in Arabidopsis. This article is protected by copyright.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QK Botany | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Plants -- Effect of cadmium on, Cadmium -- Toxicology, Arabidopsis thaliana, Genetic transcription -- Regulation, Plant cell walls | ||||||||||||||||||
Journal or Publication Title: | Journal of Integrative Plant Biology | ||||||||||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||||||||
ISSN: | 1672-9072 | ||||||||||||||||||
Official Date: | October 2023 | ||||||||||||||||||
Dates: |
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Volume: | 65 | ||||||||||||||||||
Number: | 10 | ||||||||||||||||||
Page Range: | pp. 2262-2278 | ||||||||||||||||||
DOI: | 10.1111/jipb.13557 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Re-use Statement: | This is the pre-peer reviewed version of the following article: Han, G.H., Huang, R.N., Hong, L.H., Xu, J.X., Hong, Y.G., Wu, Y.H., and Chen, W.W. (2023). The transcription factor NAC102 confers cadmium tolerance by regulating WAKL11 expression and cell wall pectin metabolism in Arabidopsis. J. Integr. Plant Biol., which has been published in final form at https://doi.org/10.1111/jipb.13557. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 27 September 2023 | ||||||||||||||||||
Date of first compliant Open Access: | 27 September 2023 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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