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Structural and biophysical characterization of the Borna disease virus 1 phosphoprotein
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Whitehead, Jack D., Grimes, Jonathan M. and Keown, Jeremy R. (2023) Structural and biophysical characterization of the Borna disease virus 1 phosphoprotein. Acta Crystallographica Section F Structural Biology Communications, 79 (3). pp. 51-60. doi:10.1107/S2053230X23000717 ISSN 2053-230X.
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Official URL: http://doi.org/10.1107/S2053230X23000717
Abstract
Bornaviruses are RNA viruses with a mammalian, reptilian, and avian host range. The viruses infect neuronal cells and in rare cases cause a lethal encephalitis. The family Bornaviridae are part of the Mononegavirales order of viruses, which contain a nonsegmented viral genome. Mononegavirales encode a viral phosphoprotein (P) that binds both the viral polymerase (L) and the viral nucleoprotein (N). The P protein acts as a molecular chaperone and is required for the formation of a functional replication/transcription complex. In this study, the structure of the oligomerization domain of the phosphoprotein determined by X-ray crystallography is reported. The structural results are complemented with biophysical characterization using circular dichroism, differential scanning calorimetry and small-angle X-ray scattering. The data reveal the phosphoprotein to assemble into a stable tetramer, with the regions outside the oligomerization domain remaining highly flexible. A helix-breaking motif is observed between the α-helices at the midpoint of the oligomerization domain that appears to be conserved across the Bornaviridae. These data provide information on an important component of the bornavirus replication complex.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Acta Crystallographica Section F Structural Biology Communications | ||||||
Publisher: | International Union of Crystallography | ||||||
ISSN: | 2053-230X | ||||||
Official Date: | 1 March 2023 | ||||||
Dates: |
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Volume: | 79 | ||||||
Number: | 3 | ||||||
Page Range: | pp. 51-60 | ||||||
DOI: | 10.1107/S2053230X23000717 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) |
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