The Library
Structural analysis of the menangle virus protein reveals a soft boundary between ordered and disordered regions
Tools
Webby, Melissa N., Herr, Nicole, Bulloch, Esther M. M., Schmitz, Michael, Keown, Jeremy R., Goldstone, David C. and Kingston, Richard L. (2021) Structural analysis of the menangle virus protein reveals a soft boundary between ordered and disordered regions. Viruses , 13 (9). 1737. doi:10.3390/v13091737 ISSN 1999-4915.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://doi.org/10.3390/v13091737
Abstract
The paramyxoviral phosphoprotein (P protein) is the non-catalytic subunit of the viral RNA polymerase, and coordinates many of the molecular interactions required for RNA synthesis. All paramyxoviral P proteins oligomerize via a centrally located coiled-coil that is connected to a downstream binding domain by a dynamic linker. The C-terminal region of the P protein coordinates interactions between the catalytic subunit of the polymerase, and the viral nucleocapsid housing the genomic RNA. The inherent flexibility of the linker is believed to facilitate polymerase translocation. Here we report biophysical and structural characterization of the C-terminal region of the P protein from Menangle virus (MenV), a bat-borne paramyxovirus with zoonotic potential. The MenV P protein is tetrameric but can dissociate into dimers at sub-micromolar protein concentrations. The linker is globally disordered and can be modeled effectively as a worm-like chain. However, NMR analysis suggests very weak local preferences for alpha-helical and extended beta conformation exist within the linker. At the interface between the disordered linker and the structured C-terminal binding domain, a gradual disorder-to-order transition occurs, with X-ray crystallographic analysis revealing a dynamic interfacial structure that wraps the surface of the binding domain.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Viruses | ||||||
Publisher: | MDPI AG | ||||||
ISSN: | 1999-4915 | ||||||
Official Date: | 31 August 2021 | ||||||
Dates: |
|
||||||
Volume: | 13 | ||||||
Number: | 9 | ||||||
Article Number: | 1737 | ||||||
DOI: | 10.3390/v13091737 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |