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A method for analyzing the composition of viral nucleoprotein complexes, produced by heterologous expression in bacteria
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Webby, Melissa N., Sullivan, Matthew P., Yegambaram, Kavestri M., Radjainia, Mazdak, Keown, Jeremy R. and Kingston, Richard L. (2019) A method for analyzing the composition of viral nucleoprotein complexes, produced by heterologous expression in bacteria. Virology, 527 . pp. 159-168. doi:10.1016/j.virol.2018.11.013 ISSN 0042-6822.
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Official URL: http://doi.org/10.1016/j.virol.2018.11.013
Abstract
Viral genomes are protected and organized by virally encoded packaging proteins. Heterologous production of these proteins often results in formation of particles resembling the authentic viral capsid or nucleocapsid, with cellular nucleic acids packaged in place of the viral genome. Quantifying the total protein and nucleic acid content of particle preparations is a recurrent biochemical problem. We describe a method for resolving this problem, developed when characterizing particles resembling the Menangle Virus nucleocapsid. The protein content was quantified using the biuret assay, which is largely independent of amino acid composition. Bound nucleic acids were quantified by determining the phosphorus content, using inductively coupled plasma mass spectrometry. Estimates for the amount of RNA packaged within the particles were consistent with the structurally-characterized packaging mechanism. For a bacterially-produced nucleoprotein complex, phosphorus usually provides a unique elemental marker of bound nucleic acids, hence this method of analysis should be routinely applicable.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Virology | ||||||
Publisher: | Academic Press | ||||||
ISSN: | 0042-6822 | ||||||
Official Date: | January 2019 | ||||||
Dates: |
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Volume: | 527 | ||||||
Page Range: | pp. 159-168 | ||||||
DOI: | 10.1016/j.virol.2018.11.013 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published |
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