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Phase imaging of human cells using digital holographic microscopy
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Bryanston-Cross, Peter J., Zhao, Yikun, Judge, Thomas, Wang, Zuobin and Chen, Lujie (2023) Phase imaging of human cells using digital holographic microscopy. In: 2023 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), Chengdu, China, 31 Jul - 04 Aug 2023. Published in: 2023 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) ISBN 9798350344486. doi:10.1109/3m-nano58613.2023.10305336 ISSN 2694-510X.
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Official URL: http://doi.org/10.1109/3m-nano58613.2023.10305336
Abstract
Digital holographic microscopy (DHM) is a 3D method for imaging cells without using labels. It gives detailed information about cell shape and behavior. This study has explored two applications of 3D phase imaging. Firstly, an image was made of an out-of-focus mono chromatically illuminated cheek cell with a conventional light field microscope. The resulting Fresnel diffraction pattern associated with the cell images has been numerically refocused at various positions. The images show details of the organelles. the changing shape of the cell nucleus and the chromosomes within it. Secondly, a simple in-line DHM system used phase imaging to analyze cancer cells. The system uses a 532nm laser, 10x and 40x microscope lenses, and a CCD camera to achieve clear phase images with 250nm resolution and 23. 63x magnification. The system was used to view both the growth of cancer cells (SMMC-7721) and in a subsequent test the ability of fluorouracil, an anti-cancer drug, to destroy them. The DHM data displayed cell changes over at 24-hour intervals over a 24-96-hours period. Holographic Amplitude images show the shape of the cell and its nucleus, while phase images gave 3D cell structural details.
Item Type: | Conference Item (Paper) | ||||||||||||||||||
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Subjects: | R Medicine > R Medicine (General) | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Digital holographic microscopy, Imaging systems in medicine, Biomedical engineering, Three-dimensional imaging in medicine | ||||||||||||||||||
Journal or Publication Title: | 2023 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) | ||||||||||||||||||
Publisher: | IEEE | ||||||||||||||||||
ISBN: | 9798350344486 | ||||||||||||||||||
ISSN: | 2694-510X | ||||||||||||||||||
Official Date: | 7 November 2023 | ||||||||||||||||||
Dates: |
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DOI: | 10.1109/3m-nano58613.2023.10305336 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Re-use Statement: | © 2023 Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. | ||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||
Date of first compliant deposit: | 11 December 2023 | ||||||||||||||||||
Date of first compliant Open Access: | 11 December 2023 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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Conference Paper Type: | Paper | ||||||||||||||||||
Title of Event: | 2023 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) | ||||||||||||||||||
Type of Event: | Conference | ||||||||||||||||||
Location of Event: | Chengdu, China | ||||||||||||||||||
Date(s) of Event: | 31 Jul - 04 Aug 2023 |
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