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Detection of natural Wolbachia strains in Anopheles mosquitoes
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Walker, Thomas (2023) Detection of natural Wolbachia strains in Anopheles mosquitoes. In: Fallon, Ann M., (ed.) Wolbachia. Methods in Molecular Biology, 2739 . New York, NY: Humana, pp. 205-218. ISBN 9781071635520
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Official URL: https://doi.org/10.1007/978-1-0716-3553-7_13
Abstract
Wolbachia is an endosymbiotic bacterium that naturally infects many insect species, including mosquitoes that transmit human diseases. Wolbachia strains have been shown to inhibit the transmission of both
arboviruses and malaria Plasmodium parasites. The existence of natural strains in wild Anopheles (An.) mosquitoes, the vectors of malaria parasites, in an endosymbiotic relationship is still to be fully determined.
Although Wolbachia has been reported to be present in wild populations of the An. gambiae complex, the primary vectors of malaria in Sub-Saharan Africa, Wolbachia DNA sequence density and infection frequencies are low. As most studies have used highly sensitive nested PCR as the only detection method, more robust evidence is required to determine whether Wolbachia strains are established as endosymbionts in Anopheles species. Techniques such as fluorescent in situ hybridization, microbiome sequencing, and Wolbachia whole genome sequencing have provided concrete evidence for genuine Wolbachia strains in two mosquito species: An. moucheti and An. demeilloni. In this chapter, the current methodology used to determine if resident strains exist in Anopheles mosquitoes will be reviewed, including both PCR- and nonPCR-based protocols.
Item Type: | Book Item | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Series Name: | Methods in Molecular Biology | ||||
Publisher: | Humana | ||||
Place of Publication: | New York, NY | ||||
ISBN: | 9781071635520 | ||||
Book Title: | Wolbachia | ||||
Editor: | Fallon, Ann M. | ||||
Official Date: | 27 November 2023 | ||||
Dates: |
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Volume: | 2739 | ||||
Page Range: | pp. 205-218 | ||||
DOI: | 10.1007/978-1-0716-3553-7_13 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Copyright Holders: | © 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature |
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