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Biophysical basis of filamentous phage tactoid-mediated antibiotic tolerance in P. aeruginosa
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Böhning, Jan, Graham, Miles, Letham, Suzanne C., Davis, Luke K., Schulze, Ulrike, Stansfeld, Phillip J., Corey, Robin A., Pearce, Philip , Tarafder, Abul K. and Bharat, Tanmay A. M. (2023) Biophysical basis of filamentous phage tactoid-mediated antibiotic tolerance in P. aeruginosa. Nature Communications, 14 . 8429. doi:10.1038/s41467-023-44160-8 ISSN 2041-1723.
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Official URL: https://doi.org/10.1038/s41467-023-44160-8
Abstract
Inoviruses are filamentous phages infecting numerous prokaryotic phyla. Inoviruses can self-assemble into mesoscale structures with liquid-crystalline order, termed tactoids, which protect bacterial cells in Pseudomonas aeruginosa biofilms from antibiotics. Here, we investigate the structural, biophysical, and protective properties of tactoids formed by the P. aeruginosa phage Pf4 and Escherichia coli phage fd. A cryo-EM structure of the capsid from fd revealed distinct biochemical properties compared to Pf4. Fd and Pf4 formed tactoids with different morphologies that arise from differing phage geometries and packing densities, which in turn gave rise to different tactoid emergent properties. Finally, we showed that tactoids formed by either phage protect rod-shaped bacteria from antibiotic treatment, and that direct association with a tactoid is required for protection, demonstrating the formation of a diffusion barrier by the tactoid. This study provides insights into how filamentous molecules protect bacteria from extraneous substances in biofilms and in host-associated infections.
Item Type: | Journal Article | ||||||
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Alternative Title: | |||||||
Subjects: | Q Science > QR Microbiology | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Bacteriophages, Pseudomonas aeruginosa , Biofilms | ||||||
Journal or Publication Title: | Nature Communications | ||||||
Publisher: | Nature Publishing Group | ||||||
ISSN: | 2041-1723 | ||||||
Official Date: | 19 December 2023 | ||||||
Dates: |
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Volume: | 14 | ||||||
Article Number: | 8429 | ||||||
DOI: | 10.1038/s41467-023-44160-8 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 11 December 2023 | ||||||
Date of first compliant Open Access: | 20 December 2023 | ||||||
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