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Detection of DNA hybridization using liquid crystal based whispering gallery mode microbubble
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Fu, Yaoxin, Xu, Tianhua, Wang, Haonan, Wang, Ziyihui, Zhang, Yunfan, Jiang, Junfeng, Liu, Tiegen, Yang, Minghong, Fan, Xinyu, Zhang, Jianzhong and Kim, Chang-Seok (2023) Detection of DNA hybridization using liquid crystal based whispering gallery mode microbubble. In: SPIE/COS Photonics Asia, 2023, Beijing, China, Beijing, China, 14-16 Oct 2023. Published in: Proceedings of SPIE : Advanced Sensor Systems and Applications XIII, 12771 doi:10.1117/12.2680825 ISSN 0277-786X.
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Official URL: http://dx.doi.org/10.1117/12.2680825
Abstract
DNA detection based on DNA hybridization has wide applications in various fields, such as clinical diagnostics, food safety, and environmental monitoring, etc. At present, common DNA detection approaches include fluorescence-based microarray technique, electrochemical method and surface plasmon resonance (SPR), etc. However, these methods require additional precise equipments and relatively complicated detection process. In this work, we developed a biosensor platform based on a liquid crystal (LC)-amplified optofluidic whispering gallery mode (WGM) resonator to achieve ultra-sensitive, label-free, and quick-response DNA hybridization detection. Liquid crystal is a material with high sensitivity, rapid response, and low cost. It exhibits significant directional and positional ordering, and it is sensitive and responsive to external stimuli. LC molecules exhibit a uniform orientation on the surface of the resonator, when the surface is covered with an appropriate amount of ssDNA. Once complementary DNA and ssDNA hybridize on the surface, the homotropic orientation of the LCs will be destroyed. Due to the simultaneous interaction of the WGM and the LCs in the optofluidic resonator, changes caused by the DNA hybridization can be amplified, resulting in a shift in the resonance wavelength. In this experiment, we used the spectral wavelength shift as a sensing parameter to achieve the detection of target DNA, and a lower detection limit compared to traditional DNA detection methods was obtained. At the same time, this biosensor platform also shows good selectivity. Our research results suggest that the LC based WGM optical microcavity sensing platform can provide an ultra-sensitive, label free solution for DNA detection.
Item Type: | Conference Item (Paper) | ||||||||||||
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Subjects: | T Technology > TA Engineering (General). Civil engineering (General) T Technology > TJ Mechanical engineering and machinery |
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||||||
Library of Congress Subject Headings (LCSH): | Liquid crystals, Biosensors, DNA hybridization, Optofluidics | ||||||||||||
Journal or Publication Title: | Proceedings of SPIE : Advanced Sensor Systems and Applications XIII | ||||||||||||
Publisher: | S P I E - International Society for Optical Engineering | ||||||||||||
ISSN: | 0277-786X | ||||||||||||
Book Title: | Advanced Sensor Systems and Applications XIII | ||||||||||||
Official Date: | 27 November 2023 | ||||||||||||
Dates: |
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Volume: | 12771 | ||||||||||||
Article Number: | 1277106 | ||||||||||||
DOI: | 10.1117/12.2680825 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Re-use Statement: | Copyright (2023) Society of Photo‑Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this publication for a fee or for commercial purposes, and modification of the contents of the publication are prohibited. | ||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
Date of first compliant deposit: | 7 December 2023 | ||||||||||||
Date of first compliant Open Access: | 12 December 2023 | ||||||||||||
RIOXX Funder/Project Grant: |
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Conference Paper Type: | Paper | ||||||||||||
Title of Event: | SPIE/COS Photonics Asia, 2023, Beijing, China | ||||||||||||
Type of Event: | Conference | ||||||||||||
Location of Event: | Beijing, China | ||||||||||||
Date(s) of Event: | 14-16 Oct 2023 |
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