The Library
Screening of hydrophilic polymers reveals broad activity in protecting phages during cryopreservation
Tools
Marton, Huba L., Bhatt, Apoorva, Sagona, Antonia P., Kilbride, Peter and Gibson, Matthew I. (2024) Screening of hydrophilic polymers reveals broad activity in protecting phages during cryopreservation. Biomacromolecules, 25 (1). pp. 413-424. doi:10.1021/acs.biomac.3c01042 ISSN 1525-7797.
|
PDF
marton-et-al-2023-screening-of-hydrophilic-polymers-reveals-broad-activity-in-protecting-phages-during-cryopreservation.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (6Mb) | Preview |
|
PDF
WRAP-Screening-hydrophilic-polymers-broad-activity-protecting-phages-cryopreservation-23.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (2652Kb) |
Official URL: https://doi.org/10.1021/acs.biomac.3c01042
Abstract
Bacteriophages have many biotechnological and therapeutic applications, but as with other biologics, cryopreservation is essential for storage and distribution. Macromolecular cryoprotectants are emerging for a range of biologics, but the chemical space for polymer-mediated phage cryopreservation has not been explored. Here we screen the cryoprotective effect of a panel of polymers against five distinct phages, showing that nearly all the tested polymers provide a benefit. Exceptions were poly(methacrylic acid) and poly(acrylic acid), which can inhibit phage-infection with bacteria, making post-thaw recovery challenging to assess. A particular benefit of a polymeric cryopreservation formulation is that the polymers do not function as carbon sources for the phage hosts (bacteria) and hence do not interfere with post-thaw measurements. This work shows that phages are amenable to protection with hydrophilic polymers and opens up new opportunities for advanced formulations for future phage therapies and to take advantage of the additional functionality brought by the polymers.
Item Type: | Journal Article | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology R Medicine > R Medicine (General) |
||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Bacteriophages, Water-soluble polymers , Microorganisms -- Cryopreservation, Polymers in medicine | ||||||||||||||||||
Journal or Publication Title: | Biomacromolecules | ||||||||||||||||||
Publisher: | American Chemical Society | ||||||||||||||||||
ISSN: | 1525-7797 | ||||||||||||||||||
Official Date: | 2024 | ||||||||||||||||||
Dates: |
|
||||||||||||||||||
Volume: | 25 | ||||||||||||||||||
Number: | 1 | ||||||||||||||||||
Page Range: | pp. 413-424 | ||||||||||||||||||
DOI: | 10.1021/acs.biomac.3c01042 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Re-use Statement: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see ACS Articles on Request ].” | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 7 December 2023 | ||||||||||||||||||
Date of first compliant Open Access: | 21 December 2023 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
|
||||||||||||||||||
Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year