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Genetic manipulation of Indian mustard genotypes with WRR-gene(s) confers resistance against Albugo candida
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Nirwan, Shradha, Chatterjee, Anupriya, Cevik, Volkan, Holub, Eric B., Jones, Jonathan D. G., Tewari, Anand Kumar, Shrivastava, Neeraj, Agnihotri, Abha and Sharma, Pankaj (2024) Genetic manipulation of Indian mustard genotypes with WRR-gene(s) confers resistance against Albugo candida. Molecular Biology Reports, 51 . 199. doi:10.1007/s11033-023-09040-w ISSN 0301-4851.
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WRAP-genetic-manipulation-indian-mustard-genotypes-Holub-2024.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only until 25 January 2025. Contact author directly, specifying your specific needs. - Requires a PDF viewer. Download (3479Kb) |
Official URL: https://doi.org/10.1007/s11033-023-09040-w
Abstract
Background:
Brassica species is the second most important edible oilseed crop in India. Albugo candida (Pers.) Kuntze, a major oomycete disease of oilseed brassica causing white rust, leads to 60% yield loss globally. The prevalence of A. candida race 2 (Ac2V) that specifically infects B. juncea, coupled with limitations of conventional methods has resulted in a dearth of white rust resistance resources in cultivated varieties.
Methods and results:
In an effort to develop resistant plants, Agrobacterium mediated genetic transformation of three B. juncea genotypes viz., susceptible host var. Varuna, along with its doubled haploid mutant lines C66 and C69 (showing moderate tolerance to field isolates of A. candida) was initiated to transfer resistance genes (WRR8Sf-2 and WRR9Hi-0) identified in Arabidopsis thaliana against race Ac2V, that encode for Toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat proteins that recognize effectors of the pathogen races.
Conclusions:
Our results demonstrate that introduction of resistance genes from a tertiary gene pool by genetic transformation enhances disease resistance in B. juncea genotypes to a highly virulent Ac2V isolate.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Library of Congress Subject Headings (LCSH): | Brassica juncea -- Disease and pest resistance, Plant genetic engineering, Albugo candida | |||||||||
Journal or Publication Title: | Molecular Biology Reports | |||||||||
Publisher: | Springer | |||||||||
ISSN: | 0301-4851 | |||||||||
Official Date: | 25 January 2024 | |||||||||
Dates: |
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Volume: | 51 | |||||||||
Article Number: | 199 | |||||||||
DOI: | 10.1007/s11033-023-09040-w | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Re-use Statement: | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/s11033-023-09040-w | |||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||
Date of first compliant deposit: | 18 January 2024 | |||||||||
RIOXX Funder/Project Grant: |
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