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A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities
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Peng, Huadong, Darlington, Alexander P. S., South, Eric J., Chen, Hao-Hong, Jiang, Wei and Ledesma-Amaro, Rodrigo (2024) A molecular toolkit of cross-feeding strains for engineering synthetic yeast communities. Nature Microbiology, 9 (3). pp. 848-863. doi:10.1038/s41564-023-01596-4 ISSN 2058-5276.
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Official URL: https://doi.org/10.1038/s41564-023-01596-4
Abstract
Engineered microbial consortia often have enhanced system performance and robustness compared with single-strain biomanufacturing production platforms. However, few tools are available for generating co-cultures of the model and key industrial host Saccharomyces cerevisiae. Here we engineer auxotrophic and overexpression yeast strains that can be used to create co-cultures through exchange of essential metabolites. Using these strains as modules, we engineered two- and three-member consortia using different cross-feeding architectures. Through a combination of ensemble modelling and experimentation, we explored how cellular (for example, metabolite production strength) and environmental (for example, initial population ratio, population density and extracellular supplementation) factors govern population dynamics in these systems. We tested the use of the toolkit in a division of labour biomanufacturing case study and show that it enables enhanced and tuneable antioxidant resveratrol production. We expect this toolkit to become a useful resource for a variety of applications in synthetic ecology and biomanufacturing.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||
SWORD Depositor: | Library Publications Router | ||||||||
Journal or Publication Title: | Nature Microbiology | ||||||||
Publisher: | Nature Publishing Group | ||||||||
ISSN: | 2058-5276 | ||||||||
Official Date: | March 2024 | ||||||||
Dates: |
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Volume: | 9 | ||||||||
Number: | 3 | ||||||||
Page Range: | pp. 848-863 | ||||||||
DOI: | 10.1038/s41564-023-01596-4 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 13 March 2024 | ||||||||
Date of first compliant Open Access: | 13 March 2024 |
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