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Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis
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Whitley, Kevin D., Grimshaw, James, Roberts, David M., Karinou, Eleni, Stansfeld, Phillip J. and Holden, Séamus (2024) Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis. Nature Microbiology, 9 . pp. 1064-1074. doi:10.1038/s41564-024-01650-9 ISSN 2058-5276.
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WRAP-peptidoglycan-synthesis-drives-single-population-septal-cell-wall-synthases-during-division-Bacillus-subtilis-2024.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (5Mb) | Preview |
Official URL: http://dx.doi.org/10.1038/s41564-024-01650-9
Abstract
Bacterial cell division requires septal peptidoglycan (sPG) synthesis by the divisome complex. Treadmilling of the essential tubulin homologue FtsZ has been implicated in septal constriction, though its precise role remains unclear. Here we used live-cell single-molecule imaging of the divisome transpeptidase PBP2B to investigate sPG synthesis dynamics in Bacillus subtilis. In contrast to previous models, we observed a single population of processively moving PBP2B molecules whose motion is driven by peptidoglycan synthesis and is not associated with FtsZ treadmilling. However, despite the asynchronous motions of PBP2B and FtsZ, a partial dependence of PBP2B processivity on FtsZ treadmilling was observed. Additionally, through single-molecule counting experiments we provide evidence that the divisome synthesis complex is multimeric. Our results support a model for B. subtilis division where a multimeric synthesis complex follows a single track dependent on sPG synthesis whose activity and dynamics are asynchronous with FtsZ treadmilling.
Item Type: | Journal Article | |||||||||||||||||||||||||||||||||
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Subjects: | Q Science > QR Microbiology | |||||||||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Bacterial cell walls, Peptidoglycans -- Synthesis, Bacterial proteins, Bacillus subtilis | |||||||||||||||||||||||||||||||||
Journal or Publication Title: | Nature Microbiology | |||||||||||||||||||||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||||||||||||||||||||
ISSN: | 2058-5276 | |||||||||||||||||||||||||||||||||
Official Date: | April 2024 | |||||||||||||||||||||||||||||||||
Dates: |
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Volume: | 9 | |||||||||||||||||||||||||||||||||
Page Range: | pp. 1064-1074 | |||||||||||||||||||||||||||||||||
DOI: | 10.1038/s41564-024-01650-9 | |||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 13 March 2024 | |||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 15 March 2024 | |||||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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