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METHANE MONOOXYGENASE MUTANTS OF METHYLOSINUS-TRICHOSPORIUM CONSTRUCTED BY MARKER-EXCHANGE MUTAGENESIS
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UNSPECIFIED (1995) METHANE MONOOXYGENASE MUTANTS OF METHYLOSINUS-TRICHOSPORIUM CONSTRUCTED BY MARKER-EXCHANGE MUTAGENESIS. FEMS MICROBIOLOGY LETTERS, 127 (3). pp. 243-248. ISSN 0378-1097.
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Abstract
Methylosinus trichosporium OB3b synthesizes a soluble cytoplasmic methane monooxygenase when grown in copper-depleted medium and a membrane-bound particulate methane monooxygenase under copper-replete conditions. The genes encoding the hydroxylase component of soluble methane monooxygenase, carried on a plasmid in Escherichia coli, were insertionally inactivated using a kanamycin cassette and transferred back into M. trichosporium by conjugation. Marker-exchange mutagenesis, via a double homologous recombination event, yielded a soluble methane monooxygenase-negative mutant which grew only on methane using the particulate methane monooxygenase during copper-replete growth conditions, thus proving that the two methane oxidation systems in this methanotroph are genetically distinct.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0378-1097 | ||||
Official Date: | 1 April 1995 | ||||
Dates: |
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Volume: | 127 | ||||
Number: | 3 | ||||
Number of Pages: | 6 | ||||
Page Range: | pp. 243-248 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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