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A RAPID LUMINESCENT-PHAGE BASED MPN METHOD FOR THE ENUMERATION OF SALMONELLA-TYPHIMURIUM IN ENVIRONMENTAL-SAMPLES
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UNSPECIFIED (1993) A RAPID LUMINESCENT-PHAGE BASED MPN METHOD FOR THE ENUMERATION OF SALMONELLA-TYPHIMURIUM IN ENVIRONMENTAL-SAMPLES. LETTERS IN APPLIED MICROBIOLOGY, 16 (1). pp. 24-27. ISSN 0266-8254.
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Abstract
Materials such as soils, waters, sewage sludges and foods can contain low numbers of salmonellas. A most-probable-number (MPN) method that utilized a bioluminescent-bacteriophage is described that allowed the specific determination of as few as one Salmonella typhimurium cell/100 ml of material within 24 h. The method was developed with soil, lake water and sewage sludge inoculated with Salm. typhimurium and had an efficiency of 100% when tested against a traditional MPN method. The protocol is rapid, sensitive, inexpensive, has a low operator time compared to the traditional MPN method, allows for the repair of injured cells and is amenable to automation.
Item Type: | Journal Article | ||||
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Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology |
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Journal or Publication Title: | LETTERS IN APPLIED MICROBIOLOGY | ||||
Publisher: | BLACKWELL SCIENCE LTD | ||||
ISSN: | 0266-8254 | ||||
Official Date: | January 1993 | ||||
Dates: |
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Volume: | 16 | ||||
Number: | 1 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 24-27 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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