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THE FATE OF INTRODUCED STREPTOMYCETES, PLASMID AND PHAGE POPULATIONS IN A DYNAMIC SOIL SYSTEM
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UNSPECIFIED (1992) THE FATE OF INTRODUCED STREPTOMYCETES, PLASMID AND PHAGE POPULATIONS IN A DYNAMIC SOIL SYSTEM. JOURNAL OF GENERAL MICROBIOLOGY, 138 (Part 4). pp. 659-666. ISSN 0022-1287.
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Abstract
Populations of Streptomyces lividans and S. violaceolatus were monitored in natural soil amended with nutrients. The fates of a multicopy plasmid pIJ673, and an actinophage, KC301, were determined and the extent of gene transfer was estimated. The soil was incubated for 60 d during which time 'spent' soil was periodically removed followed by addition of fresh, uninoculated soil. Maximum numbers of bacteria and phage inoculants occurred at 15 d; this correlated with a peak in the amount of plasmid DNA detected and total numbers of transconjugants recovered. A KC301 lysogen of S. lividans was also recovered at this time. Plasmid DNA was monitored by two methods, bead-beating and SDS/heat lysis; the latter was specific for mycelium while the former released DNA from spores and mycelium. Southern blots of soil DNA only showed the presence of plasmid DNA in SDS/heat lysis extracts from 15 d and 17 d samples, whereas positive signals were obtained throughout the experiment from bead-beaten extracts. The results confirmed that a well-developed mycelium was necessary for conjugation, phage infection, multiplication and lysogeny in soil.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | JOURNAL OF GENERAL MICROBIOLOGY | ||||
Publisher: | SOC GENERAL MICROBIOLOGY | ||||
ISSN: | 0022-1287 | ||||
Official Date: | April 1992 | ||||
Dates: |
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Volume: | 138 | ||||
Number: | Part 4 | ||||
Number of Pages: | 8 | ||||
Page Range: | pp. 659-666 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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