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INDUCIBLE CELL ABLATION IN DROSOPHILA BY COLD-SENSITIVE RICIN-A CHAIN
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UNSPECIFIED (1992) INDUCIBLE CELL ABLATION IN DROSOPHILA BY COLD-SENSITIVE RICIN-A CHAIN. DEVELOPMENT, 114 (3). 681-&. ISSN 0950-1991.
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Abstract
We have developed a system for temperature-inducible killing of specific cells in the fruitfly Drosophila melanogaster. The system overcomes many of the limitations of existing cell ablation methods and is in principle applicable to any non-homeothermic eukaryote. Temperature-sensitive and cold-sensitive mutations in the ricin toxin A chain (RTA) of castor bean were generated in yeast. One cold-sensitive mutation, RAcs2, produced temperature-dependent ablation of eye cells in Drosophila when expressed under control of the eye-specific sev enhancer. At 29-degrees-C, cell death was observed within 7 hours in the developing eye and no obvious toxic effects were observed elsewhere; at 18-degrees-C, extremely low toxicity was observed. DNA sequencing of RAcs2 revealed a single amino acid substitution in the RTA active site cleft.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
Journal or Publication Title: | DEVELOPMENT | ||||
Publisher: | COMPANY OF BIOLOGISTS LTD | ||||
ISSN: | 0950-1991 | ||||
Official Date: | March 1992 | ||||
Dates: |
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Volume: | 114 | ||||
Number: | 3 | ||||
Number of Pages: | 0 | ||||
Page Range: | 681-& | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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