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COMPARISON OF PEPTIDE FRAGMENTATION WITH DIFFERENT IONIZATION TECHNIQUES - VARIATION WITH SAMPLE PREPARATION METHOD OF THE FRAGMENTATION OF LUTEINIZING-HORMONE-RELEASING HORMONE FOLLOWING PLASMA DESORPTION
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UNSPECIFIED (1992) COMPARISON OF PEPTIDE FRAGMENTATION WITH DIFFERENT IONIZATION TECHNIQUES - VARIATION WITH SAMPLE PREPARATION METHOD OF THE FRAGMENTATION OF LUTEINIZING-HORMONE-RELEASING HORMONE FOLLOWING PLASMA DESORPTION. AUSTRALIAN JOURNAL OF CHEMISTRY, 45 (2). pp. 403-416. ISSN 0004-9425.
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Abstract
The fragmentation patterns of the peptide luteinizing hormone-releasing hormone (LH-RH) measured in plasma desorption mass spectrometry (PDMS) and keV-atom bombardment have been compared with the field desorption (FD) mass spectrum. Several different methods of preparing the samples for PDMS have been investigated, including application to a nitrocellulose surface which was preadsorbed with a protein. Similar fragment ions were observed in keV-atom bombardment and in PDMS applied to nitrocellulose which had not been preadsorbed with a protein; different fragment ions have been observed in FD mass spectrometry. The PDMS fragmentation when LH-RH was applied to nitrocellulose which had been preadsorbed with thioredoxin was found to differ yet again. The differences in the fragmentation are rationalized on the basis of differences in conformational constraints.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | AUSTRALIAN JOURNAL OF CHEMISTRY | ||||
Publisher: | C S I R O PUBLICATIONS | ||||
ISSN: | 0004-9425 | ||||
Official Date: | 1992 | ||||
Dates: |
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Volume: | 45 | ||||
Number: | 2 | ||||
Number of Pages: | 14 | ||||
Page Range: | pp. 403-416 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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