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SINGLE-CHAIN RIBOSOME INACTIVATING PROTEINS FROM PLANTS DEPURINATE ESCHERICHIA-COLI 23S RIBOSOMAL-RNA
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UNSPECIFIED (1991) SINGLE-CHAIN RIBOSOME INACTIVATING PROTEINS FROM PLANTS DEPURINATE ESCHERICHIA-COLI 23S RIBOSOMAL-RNA. FEBS LETTERS, 290 (1-2). pp. 65-68. ISSN 0014-5793.
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Abstract
The rRNA N-glycosidase activities of the catalytically active A chains of the heterodimeric ribosome inactivating proteins (RIPs) ricin and abrin, the single-chain RIPs dianthin 30, dianthin 32, and the leaf and seed forms of pokeweed antiviral protein (PAP) were assayed on E. coli ribosomes. All of the single-chain RIPs were active on E. coli ribosomes as judged by the release of a 243 nucleotide fragment from the 3' end of 23S rRNA following aniline treatment of the RNA. In contrast, E. coli ribosomes were refractory to the A chains of ricin and abrin. The position of the modification of 23S rRNA by dianthin 32 was determined by primer extension and found to be A2660, which lies in a sequence that is highly conserved in all species.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Journal or Publication Title: | FEBS LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0014-5793 | ||||
Official Date: | 23 September 1991 | ||||
Dates: |
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Volume: | 290 | ||||
Number: | 1-2 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 65-68 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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