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WRR4, a broad-spectrum TIR-NB-LRR gene from Arabidopsis thaliana that confers white rust resistance in transgenic oilseed brassica crops
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Borhan, Mohammad Hossein, Holub, E. B., Kindrachuk, Colin, Omidi, Mansour, Bozorgmanesh-Frad, Ghazaleh and Rimmer, Samuel Roger (2009) WRR4, a broad-spectrum TIR-NB-LRR gene from Arabidopsis thaliana that confers white rust resistance in transgenic oilseed brassica crops. Molecular Plant Pathology, Vol.11 (No.2). pp. 283-291. doi:10.1111/j.1364-3703.2009.00599.x ISSN 1464-6722.
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Official URL: http://dx.doi.org/10.1111/J.1364-3703.2009.00599.X...
Abstract
White blister rust caused by Albugo candida (Pers.) Kuntze is a common and often devastating disease of oilseed and vegetable brassica crops worldwide. Physiological races of the parasite have been described, including races 2, 7 and 9 from Brassica juncea, B. rapa and B. oleracea, respectively, and race 4 from Capsella bursa-pastoris (the type host). A gene named WRR4 has been characterized recently from polygenic resistance in the wild brassica relative Arabidopsis thaliana (accession Columbia) that confers broad-spectrum white rust resistance (WRR) to all four of the above Al. candida races. This gene encodes a TIR-NB-LRR (Toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat) protein which, as with other known functional members in this subclass of intracellular receptor-like proteins, requires the expression of the lipase-like defence regulator, enhanced disease susceptibility 1 (EDS1). Thus, we used RNA interference-mediated suppression of EDS1 in a white rust-resistant breeding line of B. napus (transformed with a construct designed from the A. thaliana EDS1 gene) to determine whether defence signalling via EDS1 is functionally intact in this oilseed brassica. The eds1-suppressed lines were fully susceptible following inoculation with either race 2 or 7 isolates of Al. candida. We then transformed white rust-susceptible cultivars of B. juncea (susceptible to race 2) and B. napus (susceptible to race 7) with the WRR4 gene from A. thaliana. The WRR4-transformed lines were resistant to the corresponding Al. candida race for each host species. The combined data indicate that WRR4 could potentially provide a novel source of white rust resistance in oilseed and vegetable brassica crops.
Item Type: | Journal Article | ||||
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Alternative Title: | WRR4, a broad-spectrum toll-like/interleukin-1 receptor-nucleotide binding-leucine-rich repeat gene from Arabidopsis thaliana that confers white rust resistance in transgenic oilseed brassica crops | ||||
Subjects: | S Agriculture > SB Plant culture | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Warwick HRI (2004-2010) | ||||
Library of Congress Subject Headings (LCSH): | Albuginaceae -- Research, Arabidopsis thaliana -- Genetics, Oilseed plants -- Diseases and pests, Fungal diseases of plants, Plants -- Disease and pest resistance -- Genetic aspects | ||||
Journal or Publication Title: | Molecular Plant Pathology | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 1464-6722 | ||||
Official Date: | 18 November 2009 | ||||
Dates: |
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Volume: | Vol.11 | ||||
Number: | No.2 | ||||
Number of Pages: | 9 | ||||
Page Range: | pp. 283-291 | ||||
DOI: | 10.1111/j.1364-3703.2009.00599.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) |
Data sourced from Thomson Reuters' Web of Knowledge
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